Product Description: chaperone protein DnaJ
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0051085 | chaperone cofactor-dependent protein refolding |
| Process | GO:0042026 | protein refolding |
| Process | GO:0009408 | response to heat |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.23 | 0.88 |
| Trophozoite | expressed | 0.62 | 0.67 |
| Schizont | expressed | 0.08 | 0.95 |
| Gametocyte | expressed | 0.53 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0409400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.609 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1007000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U0J2
"Best" AlphaFill ligand hit: 1PS (3-pyridinium-1-ylpropane-1-sulfonate, Local RMSD=2.14) with 5AYK (Global RMSD=7.08)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100469
Most Similar Human Ortholog: Q96EY1
TM-align score: 0.42 | RMSD: 4.42
Seq Identity: 0.23 | Length: 243 / 672
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000103423 | DnaJ heat shock protein family (Hsp40) member A3 |
| ENSG00000276726 | DnaJ heat shock protein family (Hsp40) member A3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 19 | 27 | 31 |
| disruptive | 4 | 34 | 21 | 91 |
| missense | 4 | 34 | 19 | 80 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 80 | 31 | 35 |
| disruptive | 8 | 105 | 58 | 148 |
| missense | 4 | 87 | 50 | 112 |
PlasmoDB Total SNPs: 84
Non-coding: 56 | Synonymous: 19 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 672 | Molecular Weight (kDa): 75.916
PDB ID(s): None
Isoelectric Point: 9.77
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001305 | Heat shock protein DnaJ, cysteine-rich domain |
| InterPro | IPR001623 | DnaJ domain |
| InterPro | IPR002939 | Chaperone DnaJ, C-terminal |
| InterPro | IPR008971 | HSP40/DnaJ peptide-binding |
| InterPro | IPR012724 | Chaperone DnaJ |
| InterPro | IPR036410 | Heat shock protein DnaJ, cysteine-rich domain superfamily |
| InterPro | IPR036869 | Chaperone J-domain superfamily |
| PFam | PF00226 | DnaJ domain |
| PFam | PF00684 | Heat shock protein DnaJ, cysteine-rich domain |
| PFam | PF01556 | Chaperone DnaJ, C-terminal |
| Superfamily | SSF46565 | Chaperone J-domain superfamily |
| Superfamily | SSF49493 | HSP40/DnaJ peptide-binding |
| Superfamily | SSF57938 | Heat shock protein DnaJ, cysteine-rich domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9274886 | Cloning and characterization of heat shock protein DnaJ homologues fromPlasmodium falciparum and comparison with ring infected erythrocyte surfaceantigen. | Watanabe J | 10.1016/s0166-6851(97)00073-x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20487289 | Nuclear-encoded DnaJ homologue of Plasmodium falciparum interacts withreplication ori of the apicoplast genome. | Kumar A, Tanveer A, ..., Habib S | 10.1111/j.1365-2958.2009.07033.x |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 38270449 | TcZC3HTTP, a regulatory element that contributes to Trypanosoma cruzi cellproliferation | Romagnoli BAA, Lucena ACR, ..., Goldenberg S | 10.1128/spectrum.02880-23 |
| 38287338 | Genome-wide screen identifies new set of genes for improved heterologous laccaseexpression in Saccharomyces cerevisiae | Strawn G, Wong RWK, ..., Mayor T | 10.1186/s12934-024-02298-0 |