Product Description: replication protein A1, large subunit
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005662 | DNA replication factor A complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003684 | damaged DNA binding |
| Function | GO:0043565 | sequence-specific DNA binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Function | GO:0043047 | single-stranded telomeric DNA binding |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0006261 | DNA-dependent DNA replication |
| Process | GO:0000724 | double-strand break repair via homologous recombination |
| Process | GO:0051321 | meiotic cell cycle |
| Process | GO:0006289 | nucleotide-excision repair |
| Process | GO:0051052 | regulation of DNA metabolic process |
| Process | GO:0000723 | telomere maintenance |
| Process | GO:0007004 | telomere maintenance via telomerase |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.59 | 0.66 |
| Schizont | expressed | 0.53 | 0.67 |
| Gametocyte | expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0409600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.434 | MFS: -2.586 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1007200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1007200)
Modification: Disrupted | RMgm-2738
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U0J0
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=4.21) with 4B8W (Global RMSD=16.43)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101316
Most Similar Human Ortholog: I3L524
TM-align score: 0.58 | RMSD: 3.87
Seq Identity: 0.11 | Length: 105 / 1145
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132383 | replication protein A1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 52 | 38 | 60 |
| disruptive | 51 | 118 | 62 | 169 |
| missense | 23 | 78 | 48 | 127 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 103 | 42 | 81 |
| disruptive | 80 | 244 | 121 | 292 |
| missense | 36 | 168 | 89 | 196 |
PlasmoDB Total SNPs: 180
Non-coding: 100 | Synonymous: 60 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 1145 | Molecular Weight (kDa): 134.202
UniProt ID(s): Q9U0J0
PDB ID(s): None
Isoelectric Point: 7.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004365 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR013955 | Replication factor A, C-terminal |
| InterPro | IPR031657 | Replication protein A, OB domain |
| PFam | PF01336 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| PFam | PF08646 | Replication factor A, C-terminal |
| PFam | PF16900 | Replication protein A, OB domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11880371 | Plasmodium falciparum possesses a cell cycle-regulated short type replicationprotein A large subunit encoded by an unusual transcript. | Voss TS, Mini T, Jenoe P, Beck HP | 10.1074/jbc.M200100200 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 37272974 | Differential expression of meiosis and homologous recombination-related genes inthe life cycle of Trypanosoma cruzi | Cruz-Saavedra L, Caceres T, ..., Ramirez JD | 10.1007/s00436-023-07850-2 |
| 37474515 | Identification of a small-molecule inhibitor that selectively blocks DNA-bindingby Trypanosoma brucei replication protein A1 | Mukherjee A, Hossain Z, ..., Kim HS | 10.1038/s41467-023-39839-x |
| 37200372 | Comprehensive mutational analysis of the checkpoint signaling function ofRpa1/Ssb1 in fission yeast | Xu YJ, Bhadra S, ..., Nakamura TM | 10.1371/journal.pgen.1010691 |