Product Description: peptide chain release factor 2
SignalP Peptide: MMFFLLTLLFFYYNNFIYV
# Transmembrane Domains: 0
EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005762 | mitochondrial large ribosomal subunit |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.21 | 0.86 |
| Schizont | expressed | 0.27 | 0.82 |
| Gametocyte | expressed | 0.41 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0409700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -2.626 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1007300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1007300)
Modification: Disrupted | RMgm-2739
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1W0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.29 | aminoacyl-tRNA hydrolase | EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104401
Most Similar Human Ortholog: F5H4X5
TM-align score: 0.50 | RMSD: 3.13
Seq Identity: 0.34 | Length: 83 / 194
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130921 | chromosome 12 open reading frame 65 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 8 | 3 | 11 |
| disruptive | 1 | 14 | 6 | 27 |
| missense | 1 | 14 | 5 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 16 | 7 | 13 |
| disruptive | 8 | 46 | 19 | 61 |
| missense | 3 | 38 | 18 | 40 |
PlasmoDB Total SNPs: 146
Non-coding: 133 | Synonymous: 12 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 194 | Molecular Weight (kDa): 23.407
UniProt ID(s): Q8I1W0
PDB ID(s): None
Isoelectric Point: 10.55
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000352 | Peptide chain release factor class I |
| PFam | PF00472 | Peptide chain release factor class I |
| Superfamily | SSF75620 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 26946524 | Polypeptide release factors and stop codon recognition in the apicoplast andmitochondrion of Plasmodium falciparum. | Vaishya S, Kumar V, ..., Habib S | 10.1111/mmi.13369 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |