PF3D7_0409900 (ALP5b)

Genome location: Pf3D7_04_v3:461,577..464,007(-)

Genome classification: Core

Function and Localization

Product Description: actin-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005885	Arp2/3 protein complex
Component	GO:0015629	actin cytoskeleton
Function	GO:0005200	structural constituent of cytoskeleton
Process	GO:0034314	Arp2/3 complex-mediated actin nucleation
Process	GO:0007010	cytoskeleton organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.00	1.00
Trophozoite	not expressed	0.02	0.99
Schizont	possibly expressed	0.09	0.93
Gametocyte	not expressed	0.07	0.95

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0409900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.783 | MFS: -2.424 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1V9

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.56) with 1U2V (Global RMSD=3.62)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533275

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	22	8	25
disruptive	3	24	18	36
missense	3	24	18	35

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	37	14	29
disruptive	5	49	40	73
missense	5	45	37	54

PlasmoDB Total SNPs: 98

Non-coding: 89 | Synonymous: 6 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 416 | Molecular Weight (kDa): 48.501

UniProt ID(s): Q8I1V9

PDB ID(s): None

Isoelectric Point: 7.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004000	Actin family
InterPro	IPR043129	ATPase, nucleotide binding domain
PFam	PF00022	Actin family
Superfamily	SSF53067	ATPase, nucleotide binding domain