Product Description: ribosome biogenesis GTPase A, putative
SignalP Peptide: MLLNIFVLLVNINLLLC
# Transmembrane Domains: 0
EC Numbers: 3.6.5.1 (Heterotrimeric G-protein GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.51 | 0.71 |
| Schizont | expressed | 1.59 | 0.26 |
| Gametocyte | expressed | 0.93 | 0.54 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0410700
Resistome Missense Mutations: N463Y
Resistome Compounds with Missense Mutations: MMV668399
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.177 | MFS: -2.785 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1235500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U0I1
"Best" AlphaFill ligand hit: GCP (phosphomethylphosphonic acid guanylate ester, Local RMSD=0.38) with 3GEI (Global RMSD=13.95)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.1 | heterotrimeric G-protein GTPase | GDPGpp(NH)pYM-254890phosducinpertussis toxinPasteurella multocida toxinLeu-Gly-Asn repeat-enriched proteinregulator of G protein signaling proteinbetagamma subunit of the hetreotrimeric G protein |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101533
Most Similar Human Ortholog: U3KQ69
TM-align score: 0.75 | RMSD: 3.33
Seq Identity: 0.22 | Length: 261 / 904
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000148824 | mitochondrial ribosome associated GTPase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 39 | 17 | 45 |
| disruptive | 14 | 50 | 38 | 94 |
| missense | 8 | 43 | 37 | 75 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 80 | 26 | 48 |
| disruptive | 28 | 136 | 72 | 165 |
| missense | 15 | 102 | 54 | 121 |
PlasmoDB Total SNPs: 225
Non-coding: 138 | Synonymous: 40 | Nonsynonymous: 47 | Stop Codon: 0
Protein Length: 904 | Molecular Weight (kDa): 107.888
UniProt ID(s): Q9U0I1
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF01926 | GTP binding domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34749534 | The Stringent Response Inhibits 70S Ribosome Formation in Staphylococcus aureusby Impeding GTPase-Ribosome Interactions | Bennison DJ, Nakamoto JA, ..., Corrigan RM | 10.1128/mBio.02679-21 |
| 30366986 | Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA | Pausch P, Steinchen W, ..., Bange G | 10.1074/jbc.RA118.003070 |
| 35141754 | RbgA ensures the correct timing in the maturation of the 50S subunits functionalsites | Seffouh A, Trahan C, ..., Ortega J | 10.1093/nar/gkac059 |
| 34864321 | Conformational exploration of RbgA using molecular dynamics: Possibleimplications in ribosome maturation and GTPase activity in different nucleotidebound states | Upendra N, Krishnaveni S | 10.1016/j.jmgm.2021.108087 |
| 31665744 | Structural consequences of the interaction of RbgA with a 50S ribosomal subunitassembly intermediate | Seffouh A, Jain N, ..., Ortega J | 10.1093/nar/gkz770 |