About Gene List

PF3D7_0411200

Genome location: Pf3D7_04_v3:505,544..509,792(-)

Genome classification: Core

Function and Localization

Product Description: PP-loop family protein, putative

SignalP Peptide: MYLLCIFLYILSFLCFTIC

# Transmembrane Domains: 2

EC Numbers: 6.3.4.19 (tRNA(Ile)-lysidine synthetase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.12 0.94
Trophozoite expressed 0.45 0.75
Schizont expressed 0.05 0.97
Gametocyte expressed 0.65 0.65

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0411200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -2.96 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0612600)

  • Relative Growth Rate: 0.09 ± 0.05
  • Confidence: 7.20

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0H6

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=2.79) with 2E21 (Global RMSD=6.84)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.3.4.19 tRNAIle-lysidine synthase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532714

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 14 38 32 70
disruptive 36 160 72 202
missense 30 136 63 181

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 19 98 45 70
disruptive 87 322 144 308
missense 58 257 120 224

PlasmoDB Total SNPs: 200

Non-coding: 56 | Synonymous: 85 | Nonsynonymous: 59 | Stop Codon: 0

Protein Information

Protein Length: 1245 | Molecular Weight (kDa): 150.931

UniProt ID(s): Q9U0H6

PDB ID(s): None

Isoelectric Point: 10.27

Protein Domain Annotations:

Source Family ID Description
InterPro IPR011063 tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal
InterPro IPR012094 tRNA(Ile)-lysidine synthase
PFam PF01171 tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal
Superfamily SSF52402 N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895