PF3D7_0411400

Genome location: Pf3D7_04_v3:513,083..517,579(-)

Genome classification: Core

Function and Localization

Product Description: DEAD box ATP-dependent RNA helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0003724	RNA helicase activity
Process	GO:0016070	RNA metabolic process
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.23	0.89
Trophozoite	expressed	0.53	0.72
Schizont	expressed	0.07	0.96
Gametocyte	not expressed	0.52	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0411400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.146 | MFS: -3.217 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0H4

"Best" AlphaFill ligand hit: STE (stearic acid, Local RMSD=0.30) with 6P2A (Global RMSD=14.27)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533637

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	27	47	28	72
disruptive	47	148	83	199
missense	31	125	72	166

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	33	106	55	64
disruptive	93	335	136	285
missense	56	256	108	222

PlasmoDB Total SNPs: 189

Non-coding: 34 | Synonymous: 85 | Nonsynonymous: 70 | Stop Codon: 0

Protein Information

Protein Length: 1304 | Molecular Weight (kDa): 156.611

UniProt ID(s): Q9U0H4

PDB ID(s): None

Isoelectric Point: 9.74

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF69349