PF3D7_0412300

Genome location: Pf3D7_04_v3:543,378..545,746(+)

Genome classification: Core

Function and Localization

Product Description: phosphopantothenoylcysteine synthetase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.5 (Phosphopantothenate--cysteine ligase (CTP))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0004632	phosphopantothenate--cysteine ligase activity
Process	GO:0015937	coenzyme A biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.04	0.98
Trophozoite	not expressed	0.24	0.85
Schizont	not expressed	0.15	0.91
Gametocyte	not expressed	0.15	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0412300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.98 | MFS: -2.835 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0613600)

Relative Growth Rate: 0.96 ± 0.11
Confidence: 5.72

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9U0G7

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.24) with 7EDZ (Global RMSD=3.73)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.2.5	phosphopantothenate-cysteine ligase (CTP)	ATPAMPGTPCTPUTPKClCMPEDTAL-Cysphosphate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102247

Most Similar Human Ortholog: Q9HAB8

TM-align score: 0.85 | RMSD: 2.36

Seq Identity: 0.26 | Length: 289 / 540

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000127125	phosphopantothenoylcysteine synthetase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	12	8	14
disruptive	22	49	23	85
missense	22	47	23	66

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	22	10	22
disruptive	28	114	54	134
missense	27	91	39	68

PlasmoDB Total SNPs: 146

Non-coding: 89 | Synonymous: 32 | Nonsynonymous: 23 | Stop Codon: 2

Protein Information

Protein Length: 540 | Molecular Weight (kDa): 64.968

UniProt ID(s): Q9U0G7

PDB ID(s): None

Isoelectric Point: 7.32

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007085	DNA/pantothenate metabolism flavoprotein, C-terminal
InterPro	IPR035929	CoaB-like superfamily
PFam	PF04127	DNA/pantothenate metabolism flavoprotein, C-terminal
Superfamily	SSF102645	CoaB-like superfamily