Genome location: Pf3D7_04_v3:576,810..584,668(-)
Genome classification: InternalHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.00 | 1.00 |
| Trophozoite | possibly expressed | 0.00 | 1.00 |
| Schizont | not expressed | 0.00 | 1.00 |
| Gametocyte | not expressed | 0.01 | 1.00 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0412900
Resistome Missense Mutations: Y374F, N378K, S382R, S382N, Y385F
Resistome Compounds with Missense Mutations: AN13762, AN13956, Halofuginone
Resistome # Samples with Disruptive Mutations: 16 (16 missense, 16 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.74 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1V7
"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=4.86) with 5MZA (Global RMSD=6.75)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 2225
Non-coding: 328 | Synonymous: 564 | Nonsynonymous: 1325 | Stop Codon: 8
Protein Length: 2312 | Molecular Weight (kDa): 261.848
UniProt ID(s): A0A0F6P8G5, A0A0F6P929, A0A191VZ15, A0A191VZ31, A0A191VZA4, A0A191VZA7, A0A191VZE3, A0A191VZG3, A0A2P1AG41, A0A5P0ZZY0, A0A5P1A0E6, A0A5P1A0G8, A0A5P1A0H7, A0A5P1A0I5, A0A5P1A0N4, A0A5P1A1C1, A0A5P1A1C3, A0A5P1A1D5, A0A5P1A1D8, A0A5P1A1G0, A0A5P1A212, A3R6S0, A3R6T2, A3R6T4, A3R6U3, Q26033, Q8I1V7, Q9XZB8
PDB ID(s): None
Isoelectric Point: 5.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| InterPro | IPR041480 | Cysteine-rich interdomain region 1 gamma |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| PFam | PF18562 | Cysteine-rich interdomain region 1 gamma |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |