Product Description: 26S protease regulatory subunit 6B, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.8 (Transferred entry: 5.6.1.5)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031597 | cytosolic proteasome complex |
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Function | GO:0016887 | ATP hydrolysis activity |
| Process | GO:0045899 | positive regulation of RNA polymerase II transcription preinitiation complex assembly |
| Process | GO:0070682 | proteasome regulatory particle assembly |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.42 | 0.80 |
| Trophozoite | expressed | 0.90 | 0.52 |
| Schizont | expressed | 0.89 | 0.51 |
| Gametocyte | expressed | 0.56 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0413600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.191 | MFS: -2.71 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0715600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0715600)
Modification: Tagged | RMgm-5369
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1V1
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.03) with 4KOD (Global RMSD=4.43)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.8 | proteasome ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101965
Most Similar Human Ortholog: P43686
TM-align score: 0.92 | RMSD: 2.51
Seq Identity: 0.68 | Length: 385 / 392
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000013275 | proteasome 26S subunit, ATPase 4 |
| ENSG00000281221 | proteasome 26S subunit, ATPase 4 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 4 | 3 | 15 |
| disruptive | 0 | 1 | 0 | 2 |
| missense | 0 | 1 | 0 | 2 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 19 | 11 | 16 |
| disruptive | 0 | 7 | 9 | 26 |
| missense | 0 | 3 | 8 | 13 |
PlasmoDB Total SNPs: 89
Non-coding: 79 | Synonymous: 10 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 392 | Molecular Weight (kDa): 44.666
UniProt ID(s): Q8I1V1
PDB ID(s): None
Isoelectric Point: 7.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR005937 | 26S proteasome regulatory subunit P45-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032501 | Proteasomal ATPase OB C-terminal domain |
| InterPro | IPR035256 | 26S Proteasome regulatory subunit 6B |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF16450 | Proteasomal ATPase OB C-terminal domain |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |