PF3D7_0414100

Genome location: Pf3D7_04_v3:633,501..639,156(-)

Genome classification: Core

Function and Localization

Product Description: conserved Plasmodium membrane protein, unknown function

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.96
Trophozoite	not expressed	0.51	0.72
Schizont	not expressed	0.28	0.85
Gametocyte	not expressed	0.62	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0414100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.261 | MFS: -2.488 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0716100)

Relative Growth Rate: 0.65 ± 0.22
Confidence: 4.40

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0716100)

Modification: Disrupted | RMgm-2253

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1U6

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.69) with 3I5X (Global RMSD=30.85)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533621

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	20	47	22	52
disruptive	48	115	61	157
missense	39	102	57	138

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	27	85	28	66
disruptive	84	227	108	216
missense	55	189	100	172

PlasmoDB Total SNPs: 173

Non-coding: 60 | Synonymous: 53 | Nonsynonymous: 60 | Stop Codon: 0

Protein Information

Protein Length: 1485 | Molecular Weight (kDa): 178.858

UniProt ID(s): Q8I1U6

PDB ID(s): None

Isoelectric Point: 8.69

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895