PF3D7_0414500

Genome location: Pf3D7_04_v3:654,179..657,778(-)

Genome classification: Core

Function and Localization

Product Description: male development protein MD5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003676	nucleic acid binding
Process	GO:0016070	RNA metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.01	1.00
Trophozoite	not expressed	0.01	1.00
Schizont	possibly expressed	0.01	0.99
Gametocyte	expressed	0.64	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0414500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.992 | MFS: -2.762 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0716500)

Relative Growth Rate: 0.94 ± 0.11
Confidence: 5.73

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0716500)

Modification: Disrupted | RMgm-5082

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1U3

"Best" AlphaFill ligand hit: GTA (p1-7-methylguanosine-p3-adenosine-5',5'-triphosphate, Local RMSD=0.03) with 6D0Y (Global RMSD=8.25)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101082

Most Similar Human Ortholog: J3QKT5

TM-align score: 0.75 | RMSD: 2.80

Seq Identity: 0.33 | Length: 93 / 363

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000135097	musashi RNA binding protein 1
ENSG00000153944	musashi RNA binding protein 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	14	9	15
disruptive	0	8	3	24
missense	0	8	3	20

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	31	11	21
disruptive	2	34	29	68
missense	1	23	20	48

PlasmoDB Total SNPs: 292

Non-coding: 268 | Synonymous: 22 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 363 | Molecular Weight (kDa): 42.268

UniProt ID(s): Q8I1U3

PDB ID(s): None

Isoelectric Point: 8.17

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR035979	RNA-binding domain superfamily
InterPro	IPR037366	BOULE/DAZ family
PFam	PF00076	RNA recognition motif domain
Superfamily	SSF54928	RNA-binding domain superfamily