PF3D7_0414700

Genome location: Pf3D7_04_v3:660,048..663,084(+)

Genome classification: Core

Function and Localization

Product Description: GTP-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Function	GO:0005525	GTP binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.14	0.93
Trophozoite	expressed	0.68	0.62
Schizont	expressed	0.87	0.51
Gametocyte	possibly expressed	0.49	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0414700

Resistome Missense Mutations: N280K

Resistome Compounds with Missense Mutations: MMV019662

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.951 | MFS: -0.11 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0716700)

Relative Growth Rate: 0.58 ± 0.29
Confidence: 3.89

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0716700)

Modification: Disrupted | RMgm-2258

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1U1

"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.05) with 3A1U (Global RMSD=8.68)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156397

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	29	17	34
disruptive	13	43	20	58
missense	10	37	17	44

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	66	20	37
disruptive	24	90	62	127
missense	14	63	46	83

PlasmoDB Total SNPs: 96

Non-coding: 61 | Synonymous: 23 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 631 | Molecular Weight (kDa): 73.941

UniProt ID(s): Q8I1U1

PDB ID(s): None

Isoelectric Point: 10.08

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006073	GTP binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR041623	NOG1, N-terminal helical domain
PFam	PF01926	GTP binding domain
PFam	PF17835	NOG1, N-terminal helical domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895