Product Description: ATPase GET3
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.-.- (Acting on acid anhydrides.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0043529 | GET complex |
| Component | GO:0005829 | cytosol |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0016020 | membrane |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0015446 | ATPase-coupled arsenite transmembrane transporter activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006811 | ion transport |
| Process | GO:0006620 | posttranslational protein targeting to endoplasmic reticulum membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.07 | 0.95 |
| Schizont | possibly expressed | 0.02 | 0.98 |
| Gametocyte | possibly expressed | 0.03 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0415000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.158 | MFS: -3.251 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0717000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0717000)
Modification: Disrupted | RMgm-2259
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1T8
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.19) with 3IQW (Global RMSD=2.70)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.-.- | Ex: CDP-glycerol diphosphatase | ATPADPGTPCTPUTPKClKCNITPNaFTTPAMPNMN... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100709
Most Similar Human Ortholog: O43681
TM-align score: 0.85 | RMSD: 2.51
Seq Identity: 0.48 | Length: 321 / 379
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000198356 | guided entry of tail-anchored proteins factor 3, ATPase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 18 | 6 | 23 |
| disruptive | 0 | 11 | 6 | 24 |
| missense | 0 | 11 | 6 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 45 | 18 | 17 |
| disruptive | 3 | 48 | 27 | 70 |
| missense | 2 | 32 | 20 | 31 |
PlasmoDB Total SNPs: 45
Non-coding: 23 | Synonymous: 21 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 379 | Molecular Weight (kDa): 43.317
UniProt ID(s): Q8I1T8
PDB ID(s): None
Isoelectric Point: 4.79
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR016300 | Arsenical pump ATPase, ArsA/GET3 |
| InterPro | IPR025723 | Anion-transporting ATPase-like domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR027542 | Arsenical pump ATPase, ArsA/GET3, eukaryotic |
| PFam | PF02374 | Anion-transporting ATPase-like domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34780541 | A conserved guided entry of tail-anchored pathway is involved in the traffickingof a subset of membrane proteins in Plasmodium falciparum. | Kumar T, Maitra S, Rahman A, Bhattacharjee S | 10.1371/journal.ppat.1009595 |
| 34887561 | Structural insights into metazoan pretargeting GET complexes | Keszei AFA, Yip MCJ, Hsieh TC, Shao S | 10.1038/s41594-021-00690-7 |
| 33542241 | Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2in yeast | Zhang Y, De Laurentiis E, ..., Rospert S | 10.1038/s41467-021-20981-3 |
| 37882342 | Proteome-wide abundance profiling of yeast deletion strains for GET pathwaymembers using sample multiplexing | Gygi JS, Liu X, Levi BP, Paulo JA | 10.1002/pmic.202300303 |
| 37286562 | The GET pathway is a major bottleneck for maintaining proteostasis inSaccharomyces cerevisiae | Josefson R, Kumar N, ..., Nystrom T | 10.1038/s41598-023-35666-8 |
| 33741343 | J-domain proteins promote client relay from Hsp70 during tail-anchored membraneprotein targeting | Cho H, Shim WJ, Liu Y, Shan SO | 10.1016/j.jbc.2021.100546 |