PF3D7_0415100

Genome location: Pf3D7_04_v3:670,824..672,750(+)

Genome classification: Core

Function and Localization

Product Description: 25S rRNA (uridine-N(3))-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0070042	rRNA (uridine-N3-)-methyltransferase activity
Process	GO:0070475	rRNA base methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.41	0.81
Trophozoite	possibly expressed	0.34	0.79
Schizont	expressed	0.08	0.95
Gametocyte	possibly expressed	0.39	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0415100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.133 | MFS: -2.806 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1T7

"Best" AlphaFill ligand hit: PAM (palmitoleic acid, Local RMSD=13.30) with 3GBG (Global RMSD=16.04)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105097

Most Similar Human Ortholog: E9PK62

TM-align score: 0.54 | RMSD: 2.26

Seq Identity: 0.19 | Length: 69 / 413

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000255561	ferredoxin-fold anticodon binding domain containing 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	25	6	15
disruptive	8	23	17	53
missense	4	17	14	37

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	35	8	21
disruptive	12	98	35	132
missense	4	55	28	67

PlasmoDB Total SNPs: 33

Non-coding: 7 | Synonymous: 20 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 413 | Molecular Weight (kDa): 49.186

UniProt ID(s): Q8I1T7

PDB ID(s): None

Isoelectric Point: 9.54

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR019446	Domain of unknown function DUF2431
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF10354	Domain of unknown function DUF2431
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase