PF3D7_0415400

Genome location: Pf3D7_04_v3:686,017..687,474(-)

Genome classification: Core

Function and Localization

Product Description: coatomer subunit zeta, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030126	COPI vesicle coat
Process	GO:0006891	intra-Golgi vesicle-mediated transport
Process	GO:0006886	intracellular protein transport
Process	GO:0006890	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.79	0.62
Trophozoite	expressed	0.70	0.60
Schizont	expressed	0.67	0.59
Gametocyte	expressed	0.57	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0415400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.13 | MFS: -3.262 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0717400)

Relative Growth Rate: 0.20 ± 0.48
Confidence: 2.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1T3

"Best" AlphaFill ligand hit: B3P (2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol, Local RMSD=3.10) with 5NFV (Global RMSD=17.89)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101375

Most Similar Human Ortholog: J3QQQ5

TM-align score: 0.96 | RMSD: 1.00

Seq Identity: 0.28 | Length: 79 / 208

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000005243	COPI coat complex subunit zeta 2
ENSG00000111481	COPI coat complex subunit zeta 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	4	3	13
disruptive	0	5	7	16
missense	0	5	7	14

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	11	10	10
disruptive	1	15	11	27
missense	0	15	9	23

PlasmoDB Total SNPs: 109

Non-coding: 96 | Synonymous: 10 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 208 | Molecular Weight (kDa): 23.791

UniProt ID(s): Q8I1T3

PDB ID(s): None

Isoelectric Point: 4.44

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011012	Longin-like domain superfamily
InterPro	IPR022775	AP complex, mu/sigma subunit
InterPro	IPR039652	Coatomer subunit zeta
PFam	PF01217	AP complex, mu/sigma subunit
Superfamily	SSF64356	Longin-like domain superfamily