PF3D7_0415500 (CBP20)

Genome location: Pf3D7_04_v3:688,057..689,439(+)

Genome classification: Core

Function and Localization

Product Description: nuclear cap-binding protein subunit 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005846	nuclear cap binding complex
Function	GO:0000339	RNA cap binding
Process	GO:0006397	mRNA processing
Process	GO:0000398	mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.40	0.80
Trophozoite	possibly expressed	0.39	0.76
Schizont	possibly expressed	0.15	0.89
Gametocyte	expressed	0.34	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0415500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.996 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0717500)

Relative Growth Rate: 0.95 ± 0.05
Confidence: 7.21

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0717500)

Modification: Disrupted | RMgm-2262

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1T2

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.73) with 1H2T (Global RMSD=1.09)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102337

Most Similar Human Ortholog: C9JQX9

TM-align score: 0.88 | RMSD: 1.61

Seq Identity: 0.42 | Length: 83 / 234

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000114503	nuclear cap binding protein subunit 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	6	6	9
disruptive	2	9	3	14
missense	2	9	3	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	14	8	16
disruptive	5	21	12	39
missense	3	15	8	24

PlasmoDB Total SNPs: 81

Non-coding: 71 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 234 | Molecular Weight (kDa): 28.004

UniProt ID(s): Q8I1T2

PDB ID(s): None

Isoelectric Point: 10.14

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR027157	Nuclear cap-binding protein subunit 2
InterPro	IPR034148	NCBP2, RNA recognition motif
InterPro	IPR035979	RNA-binding domain superfamily
PFam	PF00076	RNA recognition motif domain
Superfamily	SSF54928	RNA-binding domain superfamily