Product Description: GTP:AMP phosphotransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.4.10 (Nucleoside-triphosphate--adenylate kinase);2.7.4.3 (Adenylate kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005759 | mitochondrial matrix |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005525 | GTP binding |
| Function | GO:0004017 | adenylate kinase activity |
| Function | GO:0046899 | nucleoside triphosphate adenylate kinase activity |
| Process | GO:0006139 | nucleobase-containing compound metabolic process |
| Process | GO:0009132 | nucleoside diphosphate metabolic process |
| Process | GO:0006163 | purine nucleotide metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.22 | 0.87 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.13 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0415600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.91 | MFS: -2.385 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0717600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0717600)
Modification: Disrupted | RMgm-2263
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1T1
"Best" AlphaFill ligand hit: AP5 (bis(adenosine)-5'-pentaphosphate, Local RMSD=0.20) with 5YCD (Global RMSD=1.49)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 9 | 3 | 8 |
| disruptive | 3 | 6 | 7 | 18 |
| missense | 3 | 6 | 6 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 16 | 7 | 16 |
| disruptive | 3 | 23 | 17 | 34 |
| missense | 3 | 21 | 14 | 29 |
PlasmoDB Total SNPs: 92
Non-coding: 82 | Synonymous: 5 | Nonsynonymous: 4 | Stop Codon: 1
Protein Length: 229 | Molecular Weight (kDa): 26.738
PDB ID(s): None
Isoelectric Point: 9.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000850 | Adenylate kinase/UMP-CMP kinase |
| InterPro | IPR006259 | Adenylate kinase subfamily |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR033690 | Adenylate kinase, conserved site |
| PFam | PF00406 | N/A |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15478799 | Adenylate kinase and GTP:AMP phosphotransferase of the malarial parasitePlasmodium falciparum. Central players in cellular energy metabolism. | Ulschmid JK, Rahlfs S, Schirmer RH, Becker K | 10.1016/j.molbiopara.2004.04.001 |
| 22684067 | Expression, purification, crystallization and preliminary X-ray analysis ofPlasmodium falciparum GTP:AMP phosphotransferase. | Law AW, Lescar J, Hao Q, Kotaka M | 10.1107/S1744309112015862 |
| 22819813 | Subcellular localization of adenylate kinases in Plasmodium falciparum. | Ma J, Rahlfs S, ..., Becker K | 10.1016/j.febslet.2012.07.013 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |