Product Description: glutamyl-tRNA(Gln) amidotransferase subunit A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.5.7 (Glutaminyl-tRNA synthase (glutamine-hydrolyzing))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0030956 | glutamyl-tRNA(Gln) amidotransferase complex |
| Function | GO:0050567 | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity |
| Process | GO:0070681 | glutaminyl-tRNAGln biosynthesis via transamidation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.18 | 0.91 |
| Trophozoite | possibly expressed | 1.08 | 0.46 |
| Schizont | expressed | 1.28 | 0.41 |
| Gametocyte | not expressed | 1.49 | 0.33 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0416100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.164 | MFS: -2.831 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0718100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0718100)
Modification: Disrupted | RMgm-2266
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1S6
"Best" AlphaFill ligand hit: TYL (n-(4-hydroxyphenyl)acetamide (tylenol), Local RMSD=0.84) with 4YJI (Global RMSD=3.61)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.5.7 | glutaminyl-tRNA synthase (glutamine-hydrolysing) | ADPpuromycinATP-gammaSchloramphenicolgamma-Glu boronic acid6-diazo-5-oxonorleucineL-methionine-S-sulfoximine2'-O-(trinitrophenyl)adenosine 5'-triphosphate3'-O-(trinitrophenyl)adenosine 5'-triphosphateadenosine 5'-[beta,gamma-methylene]triphosphate3'-deoxy-3'-(L-glutaminylamino)-N,N-dimethyladenosine3'-(L-alpha-aspartylamino)-3'-deoxy-N,N-dimethyladenosine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101205
Most Similar Human Ortholog: Q9H0R6
TM-align score: 0.84 | RMSD: 2.77
Seq Identity: 0.28 | Length: 483 / 826
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130348 | glutaminyl-tRNA amidotransferase subunit QRSL1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 15 | 13 | 32 |
| disruptive | 22 | 53 | 20 | 68 |
| missense | 14 | 40 | 18 | 52 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 42 | 16 | 43 |
| disruptive | 39 | 109 | 56 | 163 |
| missense | 20 | 74 | 42 | 93 |
PlasmoDB Total SNPs: 105
Non-coding: 39 | Synonymous: 48 | Nonsynonymous: 18 | Stop Codon: 0
Protein Length: 826 | Molecular Weight (kDa): 96.15
UniProt ID(s): Q8I1S6
PDB ID(s): None
Isoelectric Point: 9.61
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000120 | Amidase |
| InterPro | IPR023631 | Amidase signature domain |
| InterPro | IPR036928 | Amidase signature (AS) superfamily |
| PFam | PF01425 | Amidase signature domain |
| Superfamily | SSF75304 | Amidase signature (AS) superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 26318454 | Plasmodium Apicoplast Gln-tRNAGln Biosynthesis Utilizes a Unique GatABAmidotransferase Essential for Erythrocytic Stage Parasites. | Mailu BM, Li L, ..., Gardner MJ | 10.1074/jbc.M115.655100 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 38422019 | Pathogenic GATA2 genetic variants utilize an obligate enhancer mechanism todistort a multilineage differentiation program | Katsumura KR, Liu P, ..., Bresnick EH | 10.1073/pnas.2317147121 |
| 38395946 | Multi-omics reveal mechanisms of high enteral starch diet mediated colonicdysbiosis via microbiome-host interactions in young ruminant | Jin C, Wu S, ..., Yao J | 10.1186/s40168-024-01760-w |
| 38387192 | Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bonemesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1signaling pathway | Kun W, Xiaomei C, Lei Y, Huizhi Z | 10.1016/j.intimp.2024.111713 |
| 38353733 | GATA family transcription factors in alga Chlamydomonas reinhardtii | Virolainen PA, Chekunova EM | 10.1007/s00294-024-01280-y |