PF3D7_0416300 (MCM9)

Genome location: Pf3D7_04_v3:710,117..715,849(-)

Genome classification: Core

Function and Localization

Product Description: DNA helicase MCM9, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0042555	MCM complex
Component	GO:0005634	nucleus
Function	GO:0008094	ATP-dependent activity, acting on DNA
Function	GO:0003688	DNA replication origin binding
Function	GO:0003697	single-stranded DNA binding
Process	GO:0006270	DNA replication initiation
Process	GO:0000724	double-strand break repair via homologous recombination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.51	0.70
Schizont	possibly expressed	0.40	0.76
Gametocyte	expressed	0.34	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0416300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.279 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0718300)

Relative Growth Rate: 0.97 ± 0.07
Confidence: 6.81

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0718300)

Modification: Disrupted | RMgm-2267

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1S4

"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.07) with 6MII (Global RMSD=2.47)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156438

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	25	17	47
disruptive	20	96	51	146
missense	10	75	45	124

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	55	44	62
disruptive	49	183	98	239
missense	23	143	81	183

PlasmoDB Total SNPs: 180

Non-coding: 89 | Synonymous: 49 | Nonsynonymous: 42 | Stop Codon: 0

Protein Information

Protein Length: 1465 | Molecular Weight (kDa): 171.313

UniProt ID(s): Q8I1S4

PDB ID(s): None

Isoelectric Point: 5.76

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001208	MCM domain
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR031327	Mini-chromosome maintenance protein
InterPro	IPR033762	MCM OB domain
InterPro	IPR041562	MCM, AAA-lid domain
PFam	PF00493	MCM domain
PFam	PF17207	MCM OB domain
PFam	PF17855	MCM, AAA-lid domain
Superfamily	SSF50249	Nucleic acid-binding, OB-fold
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase