Product Description: mRNA-binding protein PUF2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0017148 | negative regulation of translation |
| Process | GO:0010608 | posttranscriptional regulation of gene expression |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 1.00 |
| Trophozoite | not expressed | 0.01 | 1.00 |
| Schizont | not expressed | 0.01 | 1.00 |
| Gametocyte | possibly expressed | 0.17 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0417100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.504 | #Insertions: 5
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0719200)
Modification: Tagged | RMgm-1410
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1R7
"Best" AlphaFill ligand hit: DTD (dithiane diol, Local RMSD=0.97) with 3GVO (Global RMSD=2.39)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100436
Most Similar Human Ortholog: H0YC97
TM-align score: 0.80 | RMSD: 2.85
Seq Identity: 0.21 | Length: 217 / 514
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000055917 | pumilio RNA binding family member 2 |
| ENSG00000134644 | pumilio RNA binding family member 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 3 | 4 | 6 |
| disruptive | 2 | 2 | 1 | 17 |
| missense | 2 | 2 | 1 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 7 | 10 | 17 |
| disruptive | 5 | 17 | 22 | 84 |
| missense | 4 | 9 | 17 | 61 |
PlasmoDB Total SNPs: 49
Non-coding: 37 | Synonymous: 7 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 514 | Molecular Weight (kDa): 61.382
PDB ID(s): None
Isoelectric Point: 6.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001313 | Pumilio RNA-binding repeat |
| InterPro | IPR016024 | Armadillo-type fold |
| PFam | PF00806 | Pumilio RNA-binding repeat |
| Superfamily | SSF48371 | Armadillo-type fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15585133 | Characterization of PfPuf2, member of the Puf family RNA-binding proteins fromthe malaria parasite Plasmodium falciparum. | Fan Q, Li J, Kariuki M, Cui L | 10.1089/dna.2004.23.753 |
| 20197405 | The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sexdifferentiation in the malaria parasite Plasmodium falciparum. | Miao J, Li J, ..., Cui L | 10.1242/jcs.059824 |
| 30133543 | A seven-helix protein constitutes stress granules crucial for regulatingtranslation during human-to-mosquito transmission of Plasmodium falciparum. | Bennink S, von Bohl A, ..., Pradel G | 10.1371/journal.ppat.1007249 |
| 28596535 | Identification and characterization of roles for Puf1 and Puf2 proteins in theyeast response to high calcium | Haramati O, Brodov A, ..., Arava Y | 10.1038/s41598-017-02873-z |
| 28357358 | Translational Repression in Malaria Sporozoites | Turque O, Tsao T, Li T, Zhang M | 10.15698/mic2016.05.502 |
| 34614242 | Fission yeast RNA-binding proteins Puf2 and Puf4 are involved in repression offerrireductase Frp1 expression in response to iron | Beaudoin J, Normant V, ..., Labbe S | 10.1111/mmi.14829 |
| 32071154 | Fission Yeast Puf2, a Pumilio and FBF Family RNA-Binding Protein, Links StressGranules to Processing Bodies | Hsiao WY, Wang YT, Wang SW | 10.1128/MCB.00589-19 |
| 24681684 | Depletion of the Trypanosome Pumilio domain protein PUF2 or of some otheressential proteins causes transcriptome changes related to coding region length | Jha BA, Fadda A, ..., Clayton C | 10.1128/EC.00018-14 |
| 26735921 | UIS2: A Unique Phosphatase Required for the Development of Plasmodium LiverStages | Zhang M, Mishra S, ..., Nussenzweig V | 10.1371/journal.ppat.1005370 |
| 26808677 | Translational Control of UIS4 Protein of the Host-Parasite Interface Is Mediatedby the RNA Binding Protein Puf2 in Plasmodium berghei Sporozoites | Silva PA, Guerreiro A, ..., Mair GR | 10.1371/journal.pone.0147940 |