PF3D7_0418700

Genome location: Pf3D7_04_v3:840,757..843,217(+)

Genome classification: Core

Function and Localization

Product Description: RNA-binding protein NOB1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030688	preribosome, small subunit precursor
Function	GO:0004521	endoribonuclease activity
Process	GO:0030490	maturation of SSU-rRNA

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.83	0.61
Trophozoite	expressed	0.51	0.72
Schizont	expressed	0.37	0.82
Gametocyte	expressed	0.52	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0418700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.985 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0720800)

Relative Growth Rate: 0.13 ± 0.15
Confidence: 5.12

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1Q0

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101333

Most Similar Human Ortholog: H3BUR4

TM-align score: 0.77 | RMSD: 1.88

Seq Identity: 0.18 | Length: 62 / 569

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000141101	NIN1 (RPN12) binding protein 1 homolog

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	21	9	32
disruptive	69	86	44	108
missense	33	53	31	83

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	40	28	42
disruptive	92	152	62	145
missense	46	105	52	100

PlasmoDB Total SNPs: 141

Non-coding: 50 | Synonymous: 36 | Nonsynonymous: 55 | Stop Codon: 0

Protein Information

Protein Length: 569 | Molecular Weight (kDa): 66.544

UniProt ID(s): Q8I1Q0

PDB ID(s): None

Isoelectric Point: 5.03

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR014881	Nin one binding (NOB1) Zn-ribbon-like
InterPro	IPR017117	Ribonuclease Nob1, eukaryote
InterPro	IPR033411	Ribonuclease, PIN domain
InterPro	IPR036283	NOB1 zinc finger-like superfamily
InterPro	IPR039907	RNA-binding protein NOB1
PFam	PF08772	Nin one binding (NOB1) Zn-ribbon-like
PFam	PF17146	Ribonuclease, PIN domain
Superfamily	SSF144206	NOB1 zinc finger-like superfamily