About Gene List

PF3D7_0419700 (Pf34)

Genome location: Pf3D7_04_v3:872,600..875,321(+)

Genome classification: Core

Function and Localization

Product Description: apical merozoite protein

SignalP Peptide: MYGTFLKGSIFYLCIFFPFFSCEC

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0046658 anchored component of plasma membrane
Component GO:0020008 rhoptry
Component GO:1990225 rhoptry neck

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring possibly expressed 0.13 0.93
Trophozoite expressed 0.08 0.95
Schizont expressed 1.88 0.29
Gametocyte expressed 0.45 0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0419700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.315 | MFS: -3.44 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I1P1

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.06) with 4N57 (Global RMSD=41.54)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533455

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 0 0 1
disruptive 0 0 0 0
missense 0 0 0 0

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 0 0 1 0
disruptive 0 0 0 0
missense 0 0 0 0

PlasmoDB Total SNPs: 119

Non-coding: 85 | Synonymous: 21 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 325 | Molecular Weight (kDa): 38.835

UniProt ID(s): C5HET4, C5HET5, C5HET6, C5HET7, C5HET9, C5HEU0, C5HEU1, C5HEU2, C5HEU3, C5HEU4, C5HEU5, C5HEU6, C5HEU7, Q8I1P1

PDB ID(s): None

Isoelectric Point: 6.5

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16603573 Identification and stoichiometry of glycosylphosphatidylinositol-anchoredmembrane proteins of the human malaria parasite Plasmodium falciparum. Gilson PR, Nebl T, ..., Crabb BS 10.1074/mcp.M600035-MCP200
17521656 Plasmodium falciparum Pf34, a novel GPI-anchored rhoptry protein found indetergent-resistant microdomains. Proellocks NI, Kovacevic S, ..., Coppel RL 10.1016/j.ijpara.2007.03.013
19837093 Characterization and antigenicity of the promising vaccine candidate Plasmodiumvivax 34kDa rhoptry antigen (Pv34) Mongui A, Angel DI, ..., Patarroyo MA 10.1016/j.vaccine.2009.10.034
20654670 Conserved high activity binding peptides from the Plasmodium falciparum Pf34rhoptry protein inhibit merozoites in vitro invasion of red blood cells Arevalo-Pinzon G, Curtidor H, ..., Patarroyo ME 10.1016/j.peptides.2010.07.009
22024312 Identification, characterization and antigenicity of the Plasmodium vivax rhoptryneck protein 1 (PvRON1) Moreno-Perez DA, Montenegro M, Patarroyo ME, Patarroyo MA 10.1186/1475-2875-10-314
34425378 Genetic diversity and expression profile of Plasmodium falciparum Pf34 genesupports its immunogenicity Krishna S, Bhandari S, ..., Bharti PK 10.1016/j.retram.2021.103308