PF3D7_0419900

Genome location: Pf3D7_04_v3:876,213..894,383(+)

Genome classification: Core

Function and Localization

Product Description: phosphatidylinositol 4-kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 17

EC Numbers: 2.7.1.67 (1-phosphatidylinositol 4-kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0016020	membrane
Function	GO:0004430	1-phosphatidylinositol 4-kinase activity
Function	GO:0052742	phosphatidylinositol kinase activity
Process	GO:0046488	phosphatidylinositol metabolic process
Process	GO:0046854	phosphatidylinositol phosphate biosynthetic process
Process	GO:0048015	phosphatidylinositol-mediated signaling

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.83	0.32
Trophozoite	expressed	1.91	0.24
Schizont	expressed	2.26	0.24
Gametocyte	expressed	1.54	0.32

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0419900

Resistome Missense Mutations: D1117N, N1118D, E3090G

Resistome Compounds with Missense Mutations: KAE678/NITD678, Suloctidil

Resistome # Samples with Disruptive Mutations: 5 (2 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.011 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0722000)

Modification: Disrupted | RMgm-2281

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.67	1-phosphatidylinositol 4-kinase	ATPADPAMPGTPGDPCTPUTPKClNEMSDSGMPCDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532473

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	84	251	156	281
disruptive	378	845	472	1024
missense	228	659	398	803

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	126	510	210	345
disruptive	646	1756	733	1601
missense	365	1305	569	966

PlasmoDB Total SNPs: 1096

Non-coding: 298 | Synonymous: 384 | Nonsynonymous: 410 | Stop Codon: 4

Protein Information

Protein Length: 5035 | Molecular Weight (kDa): 611.666

UniProt ID(s): B9ZSJ6

PDB ID(s): None

Isoelectric Point: 9.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000403	Phosphatidylinositol 3-/4-kinase, catalytic domain
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR015433	Phosphatidylinositol kinase
InterPro	IPR018936	Phosphatidylinositol 3/4-kinase, conserved site
PFam	N/A	N/A
Superfamily	SSF56112	Protein kinase-like domain superfamily

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104