Product Description: serine/threonine protein kinase RIO2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Component | GO:0030688 | preribosome, small subunit precursor |
| Function | GO:0004672 | protein kinase activity |
| Process | GO:0030490 | maturation of SSU-rRNA |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.40 | 0.80 |
| Trophozoite | expressed | 0.39 | 0.77 |
| Schizont | expressed | 0.17 | 0.89 |
| Gametocyte | expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0420100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -2.981 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0521400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I1N8
"Best" AlphaFill ligand hit: G8H (2-naphthalen-2-yl-~{n}-pyridin-2-yl-ethanamide, Local RMSD=0.15) with 6HK6 (Global RMSD=3.97)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9BVS4 | Serine/threonine-protein kinase RIO2 | Homo sapiens | OrthoDB | CHEMBL2205766CHEMBL4777902CHEMBL4744511CHEMBL4754904... |
Ortholog Group (OrthoMCL): OG6_101547
Most Similar Human Ortholog: Q9BVS4
TM-align score: 0.57 | RMSD: 3.89
Seq Identity: 0.40 | Length: 351 / 581
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000058729 | RIO kinase 2 |
Protein Length: 581 | Molecular Weight (kDa): 68.742
UniProt ID(s): Q8I1N8
PDB ID(s): None
Isoelectric Point: 7.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000687 | RIO kinase |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR015285 | RIO2 kinase winged helix domain, N-terminal |
| InterPro | IPR030484 | Serine/threonine-protein kinase Rio2 |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| PFam | PF01163 | RIO2 kinase winged helix domain, N-terminal |
| PFam | PF09202 | |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 24 | 19 | 38 |
| disruptive | 14 | 65 | 55 | 93 |
| missense | 12 | 61 | 53 | 84 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 53 | 28 | 44 |
| disruptive | 30 | 161 | 56 | 148 |
| missense | 22 | 135 | 52 | 104 |
PlasmoDB Total SNPs: 206
Non-coding: 148 | Synonymous: 34 | Nonsynonymous: 23 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 27150807 | Cloning, Overexpression, Purification and Immunolocal-ization of PFD0975w fromthe Malaria Parasite Plasmodium falciparum. | Chakraborty A, Balaji SN, Sahasrabuddhe AA, Trivedi V | 10.2174/1871526516666160506150651 |
| 24948609 | Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40Sbiogenesis factors in 80S-like ribosomes | Ferreira-Cerca S, Kiburu I, ..., Hurt E | 10.1093/nar/gku542 |
| 35438042 | The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40Smaturation | Rossler I, Weigl S, ..., Pertschy B | 10.1080/15476286.2022.2064073 |
| 29459436 | Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit | Scaiola A, Pena C, ..., Ban N | 10.15252/embj.201798499 |
| 34934010 | Release of the ribosome biogenesis factor Bud23 from small subunit precursors inyeast | Black JJ, Johnson AW | 10.1261/rna.079025.121 |
| 25294836 | Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits | Turowski TW, Lebaron S, ..., Tollervey D | 10.1093/nar/gku878 |
| 29237037 | Insights into the evolutionary conserved regulation of Rio ATPase activity | Knuppel R, Christensen RH, ..., Ferreira-Cerca S | 10.1093/nar/gkx1236 |
| 29233188 | The RIO protein kinase-encoding gene Sj-riok-2 is involved in key reproductiveprocesses in Schistosoma japonicum | Zhao L, He X, ..., Hu M | 10.1186/s13071-017-2524-7 |
| 23104056 | ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40Sribosomal subunit | Ferreira-Cerca S, Sagar V, ..., LaRonde-LeBlanc N | 10.1038/nsmb.2403 |
| 35031584 | The modifying enzyme Tsr3 establishes the hierarchy of Rio kinase binding in 40Sribosome assembly | Huang H, Parker M, Karbstein K | 10.1261/rna.078994.121 |
| 29892951 | Genome-wide identification and characterization of the RIO atypical kinase familyin plants | Gao Q, Xu S, ..., Zhao X | 10.1007/s13258-018-0658-4 |
| 24424021 | CK1delta and CK1epsilon are components of human 40S subunit precursors required forcytoplasmic 40S maturation | Zemp I, Wandrey F, ..., Kutay U | 10.1242/jcs.138719 |
| 24211636 | Skeletal hybridization and PfRIO-2 kinase modeling for synthesis of alpha-pyroneanalogs as anti-malarial agent | Parveen A, Chakraborty A, ..., Bal C | 10.1016/j.ejmech.2013.10.028 |
| 30653518 | Impact of two neighbouring ribosomal protein clusters on biogenesis factorbinding and assembly of yeast late small ribosomal subunit precursors | Linnemann J, Poll G, ..., Milkereit P | 10.1371/journal.pone.0203415 |
| 25891899 | Discovery of selective RIO2 kinase small molecule ligand | Varin T, Godfrey AG, ..., Vieth M | 10.1016/j.bbapap.2015.04.006 |
| 31390939 | In vitro dimerization of human RIO2 kinase | Maurice F, Perebaskine N, Thore S, Fribourg S | 10.1080/15476286.2019.1653679 |
| 29155690 | Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomycescerevisiae | Heuer A, Thomson E, ..., Beckmann R | 10.7554/eLife.30189 |