About Gene List

PF3D7_0420700 (VAR)

Genome location: Pf3D7_04_v3:935,031..941,875(-)

Genome classification: InternalHypervariable

Function and Localization

Product Description: erythrocyte membrane protein 1, PfEMP1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020002 host cell plasma membrane
Component GO:0020030 infected host cell surface knob
Function GO:0050839 cell adhesion molecule binding
Process GO:0020033 antigenic variation
Process GO:0098609 cell-cell adhesion
Process GO:0020035 cytoadherence to microvasculature, mediated by symbiont protein
Process GO:0020013 modulation by symbiont of host erythrocyte aggregation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.00 1.00
Trophozoite not expressed 0.00 1.00
Schizont not expressed 0.00 1.00
Gametocyte not expressed 0.02 0.99

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0420700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.925 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IFQ6

"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.37) with 5MZA (Global RMSD=5.64)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104345

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 1643

Non-coding: 112 | Synonymous: 410 | Nonsynonymous: 1118 | Stop Codon: 3

Protein Information

Protein Length: 2152 | Molecular Weight (kDa): 247.611

UniProt ID(s): A0A0F6P926, A0A191VZ54, A0A5P1A053, A0A5P1A0G0, A0A5P1A0Y6, A0A5P1A1R9, A0A5P1A2E0, A0A5P1A2H2, A0A5P1A341, Q8IFQ6

PDB ID(s): None

Isoelectric Point: 5.2

Protein Domain Annotations:

Source Family ID Description
InterPro IPR004258 Duffy-binding-like domain
InterPro IPR008602 Duffy-antigen binding
InterPro IPR029210 Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
InterPro IPR029211 Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
InterPro IPR041480 Cysteine-rich interdomain region 1 gamma
PFam PF03011 Duffy-binding-like domain
PFam PF05424 Duffy-antigen binding
PFam PF15445 Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
PFam PF15447 Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
PFam PF18562 Cysteine-rich interdomain region 1 gamma
Superfamily SSF140924 N/A

Associated Publications

PMID Title Authors DOI/Link
9230440 P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. Rowe JA, Moulds JM, Newbold CI, Miller LH 10.1038/40888
9291676 Knob proteins in falciparum malaria. Sharma YD https://pubmed.ncbi.nlm.nih.gov/9291676/
9786187 A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. Cooke BM, Nicoll CL, Baruch DI, Coppel RL 10.1046/j.1365-2958.1998.01040.x
11316367 Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. Nogueira PA, Wunderlich G, Pereira da Silva LH 10.1016/s0923-2508(01)01184-6
11420100 Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. Craig A, Scherf A https://pubmed.ncbi.nlm.nih.gov/11420100/
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
18785843 Antigenic variation in Plasmodium falciparum. Scherf A, Lopez-Rubio JJ, Riviere L 10.1146/annurev.micro.61.080706.093134