PF3D7_0422000

Genome location: Pf3D7_04_v3:997,886..1,000,908(+)

Genome classification: Core

Function and Localization

Product Description: steroid dehydrogenase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 1.1.1.35 (3-hydroxyacyl-CoA dehydrogenase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016491	oxidoreductase activity
Process	GO:0006633	fatty acid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.04	0.98
Trophozoite	expressed	0.08	0.96
Schizont	expressed	0.03	0.98
Gametocyte	expressed	0.33	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0422000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.22 | MFS: -2.969 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0522400)

Relative Growth Rate: 0.20 ± 0.29
Confidence: 3.86

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0522400)

Modification: Disrupted | RMgm-4615

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4B9

"Best" AlphaFill ligand hit: CE1 (o-dodecanyl octaethylene glycol, Local RMSD=0.33) with 8G9V (Global RMSD=4.99)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.1.1.35	3-hydroxyacyl-CoA dehydrogenase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P70385	17-beta-hydroxysteroid dehydrogenase type 3	Mus musculus	HOGENOM, OrthoDB	CHEMBL1934493
P37058	17-beta-hydroxysteroid dehydrogenase type 3	Homo sapiens	HOGENOM, OrthoDB	17Beta-HSD Inhibitor, 3Phenyl-p-benzoquinone, 5Diphenyl-p-benzoquinone, 6CHEMBL276915...
O54939	17-beta-hydroxysteroid dehydrogenase type 3	Rattus norvegicus	OrthoDB	CHEMBL1813731CHEMBL3605211CHEMBL3605212Androsterone...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100431

Most Similar Human Ortholog: Q53GQ0

TM-align score: 0.90 | RMSD: 2.21

Seq Identity: 0.33 | Length: 307 / 321

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000103160	hydroxysteroid dehydrogenase like 1
ENSG00000149084	hydroxysteroid 17-beta dehydrogenase 12

Protein Information

Protein Length: 321 | Molecular Weight (kDa): 37.215

UniProt ID(s): C0H4B9

PDB ID(s): None

Isoelectric Point: 9.94

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002347	Short-chain dehydrogenase/reductase SDR
InterPro	IPR036291	NAD(P)-binding domain superfamily
PFam	PF00106	Short-chain dehydrogenase/reductase SDR
Superfamily	SSF51735	NAD(P)-binding domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	15	14	17
disruptive	1	14	13	34
missense	1	14	12	30

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	24	29	26
disruptive	4	57	22	86
missense	2	48	19	64

PlasmoDB Total SNPs: 215

Non-coding: 199 | Synonymous: 13 | Nonsynonymous: 3 | Stop Codon: 0