About Gene List

PF3D7_0422500 (BRR2)

Genome location: Pf3D7_04_v3:1,029,062..1,038,336(+)

Genome classification: Core

Function and Localization

Product Description: pre-mRNA-splicing helicase BRR2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0010494 cytoplasmic stress granule
Component GO:0005681 spliceosomal complex
Function GO:0003724 RNA helicase activity
Function GO:0003729 mRNA binding
Process GO:0000375 RNA splicing, via transesterification reactions
Process GO:0006417 regulation of translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 1.41 0.42
Trophozoite expressed 1.59 0.28
Schizont expressed 0.79 0.57
Gametocyte expressed 1.44 0.32

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0422500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -2.951 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1303000)

  • Relative Growth Rate: 0.89 ± 0.14
  • Confidence: 5.26

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.4.13 RNA helicase ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
O75643 U5 small nuclear ribonucleoprotein 200 kDa helicase OMA, OrthoDB CHEMBL4060106CHEMBL4065018CHEMBL4070179CHEMBL4097764...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100305

Most Similar Human Ortholog: Not available

TM-align score: 0.00 | RMSD: 0.00

Seq Identity: 0.00 | Length: / 2874

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000112249 activating signal cointegrator 1 complex subunit 3
ENSG00000144028 small nuclear ribonucleoprotein U5 subunit 200

Protein Information

Protein Length: 2874 | Molecular Weight (kDa): 337.908

UniProt ID(s): Q8IFP1

PDB ID(s): None

Isoelectric Point: 5.53

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001650 Helicase, C-terminal
InterPro IPR003593 AAA+ ATPase domain
InterPro IPR004179 Sec63 domain
InterPro IPR011545 DEAD/DEAH box helicase domain
InterPro IPR014001 Helicase superfamily 1/2, ATP-binding domain
InterPro IPR014756 Immunoglobulin E-set
InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
InterPro IPR041094 Brr2, N-terminal helicase PWI domain
PFam PF00270 DEAD/DEAH box helicase domain
PFam PF00271 Helicase, C-terminal
PFam PF02889 Sec63 domain
PFam PF18149 Brr2, N-terminal helicase PWI domain
Superfamily SSF158702 P-loop containing nucleoside triphosphate hydrolase
Superfamily SSF52540 Immunoglobulin E-set
Superfamily SSF81296

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 44 134 93 169
disruptive 106 284 162 429
missense 50 233 136 344

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 67 272 103 181
disruptive 176 580 297 599
missense 89 445 231 436

PlasmoDB Total SNPs: 369

Non-coding: 96 | Synonymous: 145 | Nonsynonymous: 128 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
30898770 An Allosteric Network for Spliceosome Activation Revealed by High-ThroughputSuppressor Analysis in Saccharomyces cerevisiae Brow DA 10.1534/genetics.119.301922
31219728 Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 andGpl1 in the fission yeast Schizosaccharomyces pombe Cipakova I, Jurcik M, ..., Cipak L 10.1080/15384101.2019.1632126