Genome location: Pf3D7_04_v3:1,029,062..1,038,336(+)
Genome classification: Core
Product Description: pre-mRNA-splicing helicase BRR2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0010494 | cytoplasmic stress granule |
Component | GO:0005681 | spliceosomal complex |
Function | GO:0003724 | RNA helicase activity |
Function | GO:0003729 | mRNA binding |
Process | GO:0000375 | RNA splicing, via transesterification reactions |
Process | GO:0006417 | regulation of translation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | expressed | 1.41 | 0.42 |
Trophozoite | expressed | 1.59 | 0.28 |
Schizont | expressed | 0.79 | 0.57 |
Gametocyte | expressed | 1.44 | 0.32 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0422500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.951 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1303000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
---|---|---|---|---|
O75643 | U5 small nuclear ribonucleoprotein 200 kDa helicase | OMA, OrthoDB | CHEMBL4060106CHEMBL4065018CHEMBL4070179CHEMBL4097764... |
Ortholog Group (OrthoMCL): OG6_100305
Most Similar Human Ortholog: Not available
TM-align score: 0.00 | RMSD: 0.00
Seq Identity: 0.00 | Length: / 2874
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000112249 | activating signal cointegrator 1 complex subunit 3 |
ENSG00000144028 | small nuclear ribonucleoprotein U5 subunit 200 |
Protein Length: 2874 | Molecular Weight (kDa): 337.908
UniProt ID(s): Q8IFP1
PDB ID(s): None
Isoelectric Point: 5.53
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR001650 | Helicase, C-terminal |
InterPro | IPR003593 | AAA+ ATPase domain |
InterPro | IPR004179 | Sec63 domain |
InterPro | IPR011545 | DEAD/DEAH box helicase domain |
InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
InterPro | IPR014756 | Immunoglobulin E-set |
InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
InterPro | IPR041094 | Brr2, N-terminal helicase PWI domain |
PFam | PF00270 | DEAD/DEAH box helicase domain |
PFam | PF00271 | Helicase, C-terminal |
PFam | PF02889 | Sec63 domain |
PFam | PF18149 | Brr2, N-terminal helicase PWI domain |
Superfamily | SSF158702 | P-loop containing nucleoside triphosphate hydrolase |
Superfamily | SSF52540 | Immunoglobulin E-set |
Superfamily | SSF81296 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 44 | 134 | 93 | 169 |
disruptive | 106 | 284 | 162 | 429 |
missense | 50 | 233 | 136 | 344 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 67 | 272 | 103 | 181 |
disruptive | 176 | 580 | 297 | 599 |
missense | 89 | 445 | 231 | 436 |
PlasmoDB Total SNPs: 369
Non-coding: 96 | Synonymous: 145 | Nonsynonymous: 128 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
30898770 | An Allosteric Network for Spliceosome Activation Revealed by High-ThroughputSuppressor Analysis in Saccharomyces cerevisiae | Brow DA | 10.1534/genetics.119.301922 |
31219728 | Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 andGpl1 in the fission yeast Schizosaccharomyces pombe | Cipakova I, Jurcik M, ..., Cipak L | 10.1080/15384101.2019.1632126 |