About Gene List

PF3D7_0422700 (EIF4A3)

Genome location: Pf3D7_04_v3:1,039,634..1,041,812(+)

Genome classification: Core

Function and Localization

Product Description: eukaryotic initiation factor 4A-III, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0071013 catalytic step 2 spliceosome
Component GO:0016281 eukaryotic translation initiation factor 4F complex
Component GO:0005730 nucleolus
Component GO:0005634 nucleus
Function GO:0003723 RNA binding
Function GO:0003724 RNA helicase activity
Function GO:0003729 mRNA binding
Function GO:0003743 translation initiation factor activity
Process GO:0006417 regulation of translation
Process GO:0006446 regulation of translational initiation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 0.18 0.91
Trophozoite expressed 0.25 0.84
Schizont expressed 0.06 0.97
Gametocyte expressed 0.15 0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0422700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.134 | MFS: -2.791 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0523100)

  • Relative Growth Rate: 0.07 ± 0.12
  • Confidence: 5.69

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IFN9

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.09) with 6L5N (Global RMSD=2.53)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.4.13 RNA helicase ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P60842 Eukaryotic initiation factor 4A-I Homo sapiens HOGENOM, OrthoDB HippuristanolCHEMBL2058177CHEMBL2058177CHEMBL2170718...
P38919 Eukaryotic initiation factor 4A-III HOGENOM, OMA, OrthoDB eIF4A3 inhibitor, 1eIF4A3 inhibitor, 2CHEMBL4070102CHEMBL4070102...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101114

Most Similar Human Ortholog: P38919

TM-align score: 0.97 | RMSD: 1.13

Seq Identity: 0.66 | Length: 384 / 390

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000141543 eukaryotic translation initiation factor 4A3

Protein Information

Protein Length: 390 | Molecular Weight (kDa): 44.8

UniProt ID(s): A0T1H4, Q8IFN9, Q8WSK8

PDB ID(s): None

Isoelectric Point: 7.57

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000629 ATP-dependent RNA helicase DEAD-box, conserved site
InterPro IPR001650 Helicase, C-terminal
InterPro IPR011545 DEAD/DEAH box helicase domain
InterPro IPR014001 Helicase superfamily 1/2, ATP-binding domain
InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFam PF00270 DEAD/DEAH box helicase domain
PFam PF00271 Helicase, C-terminal
Superfamily SSF52540 P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 21 11 16
disruptive 0 9 3 15
missense 0 9 3 10

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 10 37 9 17
disruptive 3 15 12 51
missense 3 13 9 33

PlasmoDB Total SNPs: 98

Non-coding: 82 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596
33906926 Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH Sinha A, Baumgarten S, ..., Scherf A 10.1128/mBio.00661-21