Genome location: Pf3D7_04_v3:1,039,634..1,041,812(+)
Genome classification: Core
Product Description: eukaryotic initiation factor 4A-III, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0071013 | catalytic step 2 spliceosome |
Component | GO:0016281 | eukaryotic translation initiation factor 4F complex |
Component | GO:0005730 | nucleolus |
Component | GO:0005634 | nucleus |
Function | GO:0003723 | RNA binding |
Function | GO:0003724 | RNA helicase activity |
Function | GO:0003729 | mRNA binding |
Function | GO:0003743 | translation initiation factor activity |
Process | GO:0006417 | regulation of translation |
Process | GO:0006446 | regulation of translational initiation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | expressed | 0.18 | 0.91 |
Trophozoite | expressed | 0.25 | 0.84 |
Schizont | expressed | 0.06 | 0.97 |
Gametocyte | expressed | 0.15 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0422700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -2.791 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0523100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IFN9
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.09) with 6L5N (Global RMSD=2.53)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
---|---|---|---|---|
P60842 | Eukaryotic initiation factor 4A-I | Homo sapiens | HOGENOM, OrthoDB | HippuristanolCHEMBL2058177CHEMBL2058177CHEMBL2170718... |
P38919 | Eukaryotic initiation factor 4A-III | HOGENOM, OMA, OrthoDB | eIF4A3 inhibitor, 1eIF4A3 inhibitor, 2CHEMBL4070102CHEMBL4070102... |
Ortholog Group (OrthoMCL): OG6_101114
Most Similar Human Ortholog: P38919
TM-align score: 0.97 | RMSD: 1.13
Seq Identity: 0.66 | Length: 384 / 390
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000141543 | eukaryotic translation initiation factor 4A3 |
Protein Length: 390 | Molecular Weight (kDa): 44.8
UniProt ID(s): A0T1H4, Q8IFN9, Q8WSK8
PDB ID(s): None
Isoelectric Point: 7.57
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
InterPro | IPR001650 | Helicase, C-terminal |
InterPro | IPR011545 | DEAD/DEAH box helicase domain |
InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
PFam | PF00270 | DEAD/DEAH box helicase domain |
PFam | PF00271 | Helicase, C-terminal |
Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 4 | 21 | 11 | 16 |
disruptive | 0 | 9 | 3 | 15 |
missense | 0 | 9 | 3 | 10 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 10 | 37 | 9 | 17 |
disruptive | 3 | 15 | 12 | 51 |
missense | 3 | 13 | 9 | 33 |
PlasmoDB Total SNPs: 98
Non-coding: 82 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |