Genome location: Pf3D7_04_v3:1,082,005..1,084,464(-)
Genome classification: Core
Product Description: reticulocyte binding protein homologue 5
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045177 | apical part of cell |
| Component | GO:0005923 | bicellular tight junction |
| Component | GO:0009986 | cell surface |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0043655 | host extracellular space |
| Component | GO:0032991 | protein-containing complex |
| Component | GO:0020008 | rhoptry |
| Component | GO:0034591 | rhoptry lumen |
| Function | GO:0008201 | heparin binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
| Process | GO:0051205 | protein insertion into membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.45 | 0.86 |
| Trophozoite | not expressed | 0.05 | 0.97 |
| Schizont | expressed | 0.74 | 0.73 |
| Gametocyte | not expressed | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0424100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.126 | MFS: -3.141 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IFM5
"Best" AlphaFill ligand hit: PE0 (pterine, Local RMSD=0.08) with 5Z79 (Global RMSD=16.34)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 7 | 12 | 16 |
| disruptive | 9 | 23 | 16 | 57 |
| missense | 9 | 21 | 14 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 25 | 11 | 20 |
| disruptive | 23 | 73 | 42 | 67 |
| missense | 14 | 51 | 38 | 55 |
PlasmoDB Total SNPs: 95
Non-coding: 57 | Synonymous: 22 | Nonsynonymous: 16 | Stop Codon: 0
Protein Length: 526 | Molecular Weight (kDa): 62.996
UniProt ID(s): A0A159SK44, A0A159SK99, A0A159SKS8, A0A159SKW8, A0A159SL23, A0A159SL78, A0A159SL96, A0A159SLM7, A0A159SMC8, A0A159SMR9, A0A161FQT0, A0A1B1UZE2, A0A1B1UZE4, A0A1B1UZE5, A0A346RCI1, A0A346RCJ0, A0A346RCJ2, A0A346RCJ3, A0A346RCJ4, A0A346RCK4, A0A346RCK5, A0A346RCK6, A0A346RCK9, B2L3N7, Q8IFM5
PDB ID(s): 4WAT, 5MI0, 6RCU, 7PHU, 8CDD
Isoelectric Point: 8.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR041668 | Rh5 coiled-coil domain |
| PFam | PF18515 | Rh5 coiled-coil domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 18827878 | PfRH5: a novel reticulocyte-binding family homolog of plasmodium falciparum thatbinds to the erythrocyte, and an investigation of its receptor. | Rodriguez M, Lustigman S, ..., Lobo CA | 10.1371/journal.pone.0003300 |
| 21909261 | An EGF-like protein forms a complex with PfRh5 and is required for invasion ofhuman erythrocytes by Plasmodium falciparum. | Chen L, Lopaticki S, ..., Cowman AF | 10.1371/journal.ppat.1002199 |
| 22080952 | Basigin is a receptor essential for erythrocyte invasion by Plasmodiumfalciparum. | Crosnier C, Bustamante LY, ..., Wright GJ | 10.1038/nature10606 |
| 23146673 | A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitoryantibodies that are effective across common PfRH5 genetic variants. | Bustamante LY, Bartholdson SJ, ..., Rayner JC | 10.1016/j.vaccine.2012.10.106 |
| 24133188 | Naturally acquired antibodies specific for Plasmodium falciparumreticulocyte-binding protein homologue 5 inhibit parasite growth and predictprotection from malaria. | Tran TM, Ongoiba A, ..., Crompton PD | 10.1093/infdis/jit553 |
| 24212193 | Analyses of interactions between heparin and the apical surface proteins ofPlasmodium falciparum. | Kobayashi K, Takano R, ..., Kato K | 10.1038/srep03178 |
| 24297912 | RH5-Basigin interaction plays a major role in the host tropism of Plasmodiumfalciparum. | Wanaguru M, Liu W, ..., Wright GJ | 10.1073/pnas.1320771110 |
| 25583518 | Multiprotein complex between the GPI-anchored CyRPA with PfRH5 and PfRipr iscrucial for Plasmodium falciparum erythrocyte invasion. | Reddy KS, Amlabu E, ..., Gaur D | https://pubmed.ncbi.nlm.nih.gov/25583518/ |
| 28186186 | P113 is a merozoite surface protein that binds the N terminus of Plasmodiumfalciparum RH5. | Galaway F, Drought LG, ..., Wright GJ | 10.1038/ncomms14333 |
| 28195530 | Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein Cy RPA | Chen L, Xu Y, ..., Cowman AF | 10.7554/eLife.21347 |
| 29146974 | Human Cyclophilin B forms part of a multi-protein complex during erythrocyteinvasion by Plasmodium falciparum. | Prakash P, Zeeshan M, ..., Malhotra P | 10.1038/s41467-017-01638-6 |
| 29707789 | OK/basigin expression on red blood cells varies between blood donors andcorrelates with binding of recombinant Plasmodium falciparum reticulocyte-bindingprotein homolog 5. | Ye L, Zhao F, ..., Zhu Z | 10.1111/trf.14635 |
| 29848401 | Extent and Dynamics of Polymorphism in the Malaria Vaccine Candidate Plasmodiumfalciparum Reticulocyte-Binding Protein Homologue-5 in Kalifabougou, Mali. | Ouattara A, Tran TM, ..., Plowe CV | 10.4269/ajtmh.17-0737 |
| 30542156 | Structure of Plasmodium falciparum Rh5-CyRPA-Ripr invasion complex. | Wong W, Huang R, ..., Cowman AF | 10.1038/s41586-018-0779-6 |
| 31613878 | Resurrection of the ancestral RH5 invasion ligand provides a molecularexplanation for the origin of P. falciparum malaria in humans. | Galaway F, Yu R, ..., Wright GJ | 10.1371/journal.pbio.3000490 |
| 34378982 | Human Basigin (CD147) Does Not Directly Interact with SARS-CoV-2 SpikeGlycoprotein. | Ragotte RJ, Pulido D, ..., Draper SJ | 10.1128/mSphere.00647-21 |
| 37653544 | Recent increase in low complexity polygenomic infections and sialicacid-independent invasion pathways in Plasmodium falciparum from Western Gambia | Nganyewo NN, Bojang F, ..., Amambua-Ngwa A | 10.1186/s13071-023-05929-4 |
| 37572659 | Superior antibody immunogenicity of a viral-vectored RH5 blood-stage malariavaccine in Tanzanian infants as compared to adults | Silk SE, Kalinga WF, ..., Olotu AI | 10.1016/j.medj.2023.07.003 |
| 37072430 | Plasmepsin X activates the PCRCR complex of Plasmodium falciparum by processingPfRh5 for erythrocyte invasion | Triglia T, Scally SW, ..., Cowman AF | 10.1038/s41467-023-37890-2 |
| 38123677 | The PfRCR complex bridges malaria parasite and erythrocyte during invasion | Farrell B, Alam N, ..., Higgins MK | 10.1038/s41586-023-06856-1 |
| 38406638 | Limited genetic variations of the Rh5-CyRPA-Ripr invasion complex in Plasmodiumfalciparum parasite population in selected malaria-endemic regions, Kenya | Waweru H, Kanoi BN, ..., Gitaka J | 10.3389/fitd.2023.1102265 |
| 37796723 | Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5from binding to basigin-containing membrane protein complexes | Jamwal A, Constantin CF, ..., Higgins MK | 10.7554/eLife.83681 |
| 37713419 | The dual action of human antibodies specific to Plasmodium falciparum PfRH5 andPfCyRPA: Blocking invasion and inactivating extracellular merozoites | Weiss GE, Ragotte RJ, ..., Gilson PR | 10.1371/journal.ppat.1011182 |
| 38299155 | Analyses of human vaccine-specific circulating and bone marrow-resident B cellpopulations reveal benefit of delayed vaccine booster dosing with blood-stagemalaria antigens | Barrett JR, Silk SE, ..., Nielsen CM | 10.3389/fimmu.2023.1193079 |