Genome location: Pf3D7_04_v3:1,085,641..1,091,369(-)
Genome classification: Core
Product Description: reticulocyte binding protein homologue 4
SignalP Peptide: MNKNILWITFFYFLFFLLDMYQGNDA
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045177 | apical part of cell |
| Component | GO:0020008 | rhoptry |
| Function | GO:0008201 | heparin binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.66 | 0.80 |
| Trophozoite | not expressed | 0.07 | 0.96 |
| Schizont | expressed | 0.29 | 0.89 |
| Gametocyte | not expressed | 0.06 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0424200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.964 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H496
"Best" AlphaFill ligand hit: UDP (uridine-5'-diphosphate, Local RMSD=0.02) with 5H60 (Global RMSD=17.97)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 49 | 36 | 79 |
| disruptive | 74 | 177 | 111 | 271 |
| missense | 42 | 147 | 100 | 226 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 105 | 52 | 83 |
| disruptive | 108 | 372 | 179 | 354 |
| missense | 65 | 298 | 156 | 271 |
PlasmoDB Total SNPs: 181
Non-coding: 25 | Synonymous: 80 | Nonsynonymous: 76 | Stop Codon: 0
Protein Length: 1716 | Molecular Weight (kDa): 205.847
UniProt ID(s): A0A159SJR8, A0A159SJU8, A0A159SJV5, A0A159SJW5, A0A159SJW8, A0A159SKE7, A0A159SLJ4, A0A159SLK1, A0A161F2L4, A0A164KFX7, A0A2S1Q7L8, A0A2S1Q7M1, A0A346RD93, A0A346RD99, A0A346RDC4, A0A346RDC7, C0H496, Q8MWH2, Q8MWP1, Q8MWP2, U6C8F2, U6C8T1, U6C8U7, U6C974
PDB ID(s): None
Isoelectric Point: 7.37
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16123303 | Molecular mechanism for switching of P. falciparum invasion pathways into humanerythrocytes. | Stubbs J, Simpson KM, ..., Cowman AF | 10.1126/science.1115257 |
| 17971435 | Recombinant Plasmodium falciparum reticulocyte homology protein 4 binds toerythrocytes and blocks invasion. | Gaur D, Singh S, ..., Miller LH | 10.1073/pnas.0708772104 |
| 19307208 | Antibodies to reticulocyte binding protein-like homologue 4 inhibit invasion ofPlasmodium falciparum into human erythrocytes. | Tham WH, Wilson DW, ..., Cowman AF | 10.1128/IAI.00048-09 |
| 20855594 | Complement receptor 1 is the host erythrocyte receptor for Plasmodium falciparumPfRh4 invasion ligand. | Tham WH, Wilson DW, ..., Cowman AF | 10.1073/pnas.1008151107 |
| 23028883 | The Plasmodium falciparum erythrocyte invasion ligand Pfrh4 as a target offunctional and protective human antibodies against malaria. | Reiling L, Richards JS, ..., Beeson JG | 10.1371/journal.pone.0045253 |
| 24212193 | Analyses of interactions between heparin and the apical surface proteins ofPlasmodium falciparum. | Kobayashi K, Takano R, ..., Kato K | 10.1038/srep03178 |
| 24214979 | Using mutagenesis and structural biology to map the binding site for thePlasmodium falciparum merozoite protein PfRh4 on the human immune adherencereceptor. | Park HJ, Guariento M, ..., Atkinson JP | 10.1074/jbc.M113.520346 |
| 26324715 | Characterization of Inhibitors and Monoclonal Antibodies That Modulate theInteraction between Plasmodium falciparum Adhesin PfRh4 with Its ErythrocyteReceptor Complement Receptor 1. | Lim NT, Harder MJ, ..., Tham WH | 10.1074/jbc.M115.657171 |
| 27244695 | Identification of a reticulocyte-specific binding domain of Plasmodium vivaxreticulocyte-binding protein 1 that is homologous to the PfRh4erythrocyte-binding domain. | Han JH, Lee SK, ..., Han ET | 10.1038/srep26993 |
| 38132622 | Demonstration of RNAi Yeast Insecticide Activity in Semi-Field Larvicide andAttractive Targeted Sugar Bait Trials Conducted on Aedes and Culex Mosquitoes | Stewart ATM, Mysore K, ..., Duman-Scheel M | 10.3390/insects14120950 |
| 37653544 | Recent increase in low complexity polygenomic infections and sialicacid-independent invasion pathways in Plasmodium falciparum from Western Gambia | Nganyewo NN, Bojang F, ..., Amambua-Ngwa A | 10.1186/s13071-023-05929-4 |