Genome location: Pf3D7_04_v3:1,108,275..1,112,724(+)
Genome classification: Core
Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0044538 | host cell periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0044003 | modulation by symbiont of host process |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 1.63 | 0.36 |
| Trophozoite | expressed | 0.34 | 0.82 |
| Schizont | expressed | 0.21 | 0.88 |
| Gametocyte | not expressed | 0.16 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0424500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.533 | #Insertions: 4
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IFM1
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.90) with 4BFM (Global RMSD=9.50)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 24 | 15 | 34 |
| disruptive | 13 | 47 | 17 | 60 |
| missense | 10 | 43 | 17 | 54 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 52 | 16 | 36 |
| disruptive | 23 | 97 | 64 | 145 |
| missense | 16 | 84 | 47 | 84 |
PlasmoDB Total SNPs: 211
Non-coding: 162 | Synonymous: 33 | Nonsynonymous: 16 | Stop Codon: 0
Protein Length: 622 | Molecular Weight (kDa): 74.443
UniProt ID(s): Q8IFM1
PDB ID(s): None
Isoelectric Point: 9.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23973789 | Dematin, a human erythrocyte cytoskeletal protein, is a substrate for arecombinant FIKK kinase from Plasmodium falciparum. | Brandt GS, Bailey S | 10.1016/j.molbiopara.2013.08.003 |
| 27777305 | Expansion of Lysine-rich Repeats in Plasmodium Proteins Generates NovelLocalization Sequences That Target the Periphery of the Host Erythrocyte. | Davies HM, Thalassinos K, Osborne AR | 10.1074/jbc.M116.761213 |
| 32284562 | An exported kinase family mediates species-specific erythrocyte remodelling andvirulence in human malaria. | Davies H, Belda H, ..., Treeck M | 10.1038/s41564-020-0702-4 |
| 37737226 | PerTurboID, a targeted in situ method reveals the impact of kinase deletion onits local protein environment in the cytoadhesion complex of malaria-causingparasites | Davies H, Belda H, ..., Treeck M | 10.7554/eLife.86367 |