Genome location: Pf3D7_04_v3:1,115,896..1,123,289(+)
Genome classification: Core
Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0044538 | host cell periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0016301 | kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0044003 | modulation by symbiont of host process |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.21 | 0.50 |
| Trophozoite | expressed | 0.46 | 0.79 |
| Schizont | expressed | 0.23 | 0.88 |
| Gametocyte | not expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0424700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.721 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IFL9
"Best" AlphaFill ligand hit: 919 (dcc-2036, Local RMSD=1.10) with 5G6V (Global RMSD=8.54)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P05227 | Histidine-rich protein PFHRP-II | Plasmodium falciparum | OrthoMCL BLAST | CHEMBL152635CHEMBL2111198CHEMBL152052CHEMBL346177... |
Protein Length: 1222 | Molecular Weight (kDa): 143.049
UniProt ID(s): Q01420, Q01422, Q7JPV5, Q8IFL9
PDB ID(s): None
Isoelectric Point: 7.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 42 | 15 | 42 |
| disruptive | 78 | 163 | 43 | 122 |
| missense | 48 | 97 | 30 | 76 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 44 | 66 | 21 | 44 |
| disruptive | 121 | 231 | 86 | 194 |
| missense | 71 | 142 | 51 | 121 |
PlasmoDB Total SNPs: 591
Non-coding: 171 | Synonymous: 253 | Nonsynonymous: 167 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19204093 | Polymorphisms in erythrocyte binding antigens 140 and 181 affect function andbinding but not receptor specificity in Plasmodium falciparum. | Maier AG, Baum J, ..., Cowman AF | 10.1128/IAI.01331-08 |
| 24530877 | An exported kinase (FIKK4.2) that mediates virulence-associated changes inPlasmodium falciparum-infected red blood cells. | Kats LM, Fernandez KM, ..., Cooke BM | 10.1016/j.ijpara.2014.01.003 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 32284562 | An exported kinase family mediates species-specific erythrocyte remodelling andvirulence in human malaria. | Davies H, Belda H, ..., Treeck M | 10.1038/s41564-020-0702-4 |