Product Description: rhoptry-associated protein 3
SignalP Peptide: MIRKFLISLFLIFLCLNNVVIG
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Function | GO:0003723 | RNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.04 | 0.98 |
| Schizont | expressed | 1.71 | 0.31 |
| Gametocyte | not expressed | 0.02 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0501500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -2.948 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1101400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1101400)
Modification: Tagged | RMgm-210
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I485
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 3 | 3 | 13 |
| disruptive | 6 | 19 | 10 | 28 |
| missense | 6 | 19 | 8 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 8 | 8 | 17 |
| disruptive | 14 | 39 | 18 | 61 |
| missense | 10 | 28 | 15 | 41 |
PlasmoDB Total SNPs: 52
Non-coding: 35 | Synonymous: 9 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 19266084 | Identification of rhoptry trafficking determinants and evidence for a novelsorting mechanism in the malaria parasite Plasmodium falciparum. | Richard D, Kats LM, ..., Coppel RL | 10.1371/journal.ppat.1000328 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 21226723 | Antibodies to the Plasmodium falciparum rhoptry protein RAP-2/RSP-2 in relationto anaemia in Cameroonian children | Awah N, Balogun H, ..., Berzins K | 10.1111/j.1365-3024.2010.01259.x |
| 17624849 | Members of the low-molecular-mass rhoptry protein complex of Plasmodiumfalciparum bind to the surface of normal erythrocytes | Sterkers Y, Scheidig C, ..., Scherf A | 10.1086/519685 |
| 9423886 | Analysis of inhibitory epitopes in the Plasmodium falciparum rhoptry proteinRAP-1 including identification of a second inhibitory epitope | Howard RF, Jacobson KC, Rickel E, Thurman J | 10.1128/IAI.66.1.380-386.1998 |
| 9574915 | Analysis of the processing of Plasmodium falciparum rhoptry-associated protein 1and localization of Pr86 to schizont rhoptries and p67 to free merozoites | Howard RF, Narum DL, Blackman M, Thurman J | 10.1016/s0166-6851(97)00238-7 |
| 12183575 | Identification and disruption of the gene encoding the third member of thelow-molecular-mass rhoptry complex in Plasmodium falciparum | Baldi DL, Good R, ..., Cowman AF | 10.1128/IAI.70.9.5236-5245.2002 |
| 10835342 | RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodiumfalciparum | Baldi DL, Andrews KT, ..., Cowman AF | 10.1093/emboj/19.11.2435 |
| 1477099 | Three matrix metalloproteinases form a non-covalent association with therhoptry-associated protein-1 of Plasmodium falciparum | Locher CP, Vandekerckhove F, Tam LQ | 10.1016/0167-4838(92)90088-u |
| 19695213 | Mechanisms of malarial anaemia: potential involvement of the Plasmodiumfalciparum low molecular weight rhoptry-associated proteins | Awah NW, Troye-Blomberg M, Berzins K, Gysin J | 10.1016/j.actatropica.2009.08.017 |
| 37509080 | Carboxymethylated Rhizoma alismatis Polysaccharides Regulate Calcium OxalateCrystals Growth and Reduce the Regulated Crystals' Cytotoxicity | Cheng XY, Ouyang JM | 10.3390/biom13071044 |
| 23506095 | Immunoproteomic analysis of Plasmodium falciparum antigens using sera frompatients with clinical history of imported malaria | Costa RM, Nogueira F, ..., Silva MS | 10.1186/1475-2875-12-100 |
| 20833215 | Conserved regions of the Plasmodium falciparum rhoptry-associated protein 3mediate specific host-pathogen interactions during invasion of red blood cells | Garcia J, Curtidor H, ..., Patarroyo ME | 10.1016/j.peptides.2010.09.002 |