Product Description: rhoptry-associated protein 2
SignalP Peptide: MGLKFYVLVFLILCLKNVVKG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.04 | 0.98 |
| Schizont | expressed | 1.45 | 0.40 |
| Gametocyte | not expressed | 0.02 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0501600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -3.205 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1101400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1101400)
Modification: Disrupted | RMgm-4428
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I484
"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=4.83) with 1CCL (Global RMSD=19.12)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 6 | 7 | 12 |
| disruptive | 10 | 5 | 4 | 19 |
| missense | 10 | 5 | 4 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 12 | 14 | 12 |
| disruptive | 11 | 23 | 14 | 55 |
| missense | 10 | 17 | 13 | 46 |
PlasmoDB Total SNPs: 53
Non-coding: 33 | Synonymous: 8 | Nonsynonymous: 11 | Stop Codon: 1
Protein Length: 398 | Molecular Weight (kDa): 46.739
UniProt ID(s): B9VGG5, B9VGG6, C5HEW2, C5HEW3, C5HEW4, C5HEW5, C5HEW6, C5HEW7, Q26004, Q8I484, Q9TVT9, Q9U432, Q9U433
PDB ID(s): None
Isoelectric Point: 9.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 1542308 | The 42-kilodalton rhoptry-associated protein of Plasmodium falciparum. | Saul A, Cooper J, ..., Limpaiboon T | 10.1016/0166-6851(92)90251-e |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 29352039 | Disrupting CD147-RAP2 interaction abrogates erythrocyte invasion by Plasmodiumfalciparum. | Zhang MY, Zhang Y, ..., Chen ZN | 10.1182/blood-2017-08-802918 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 37509080 | Carboxymethylated Rhizoma alismatis Polysaccharides Regulate Calcium OxalateCrystals Growth and Reduce the Regulated Crystals' Cytotoxicity | Cheng XY, Ouyang JM | 10.3390/biom13071044 |
| 36107348 | Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomycescerevisiae | Lavilla-Puerta M, Giuntoli B | 10.1007/978-1-0716-2667-2_14 |