Product Description: merozoite surface protein 8
SignalP Peptide: MVFKSSYIFFFLFLVILYFNNVVEG
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0020020 | food vacuole |
| Component | GO:0016020 | membrane |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0020003 | symbiont-containing vacuole |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 2.09 | 0.23 |
| Trophozoite | possibly expressed | 0.35 | 0.82 |
| Schizont | not expressed | 0.26 | 0.85 |
| Gametocyte | not expressed | 0.55 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0502400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.666 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1102200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1102200)
Modification: Disrupted | RMgm-2925
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I476
"Best" AlphaFill ligand hit: TMA (tetramethylammonium ion, Local RMSD=0.32) with 4YLQ (Global RMSD=14.91)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 41 | 18 | 33 |
| disruptive | 60 | 160 | 65 | 130 |
| missense | 34 | 108 | 54 | 99 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 69 | 21 | 42 |
| disruptive | 107 | 257 | 113 | 235 |
| missense | 61 | 178 | 87 | 133 |
PlasmoDB Total SNPs: 171
Non-coding: 40 | Synonymous: 63 | Nonsynonymous: 68 | Stop Codon: 0
Protein Length: 597 | Molecular Weight (kDa): 69.448
UniProt ID(s): C5HDU3, Q8I476, Q95NL3, Q964F5, Q964F6, Q964F7, Q964F8, S5Y6C6, S5Y6E5, S5YCY1, S5YCZ8, S5YJI2, S5YX06
PDB ID(s): None
Isoelectric Point: 4.43
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR024731 | EGF domain |
| PFam | PF12947 | EGF domain |
| Superfamily | SSF57196 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11378201 | Merozoite surface protein 8 of Plasmodium falciparum contains two epidermalgrowth factor-like domains. | Black CG, Wu T, ..., Coppel RL | 10.1016/s0166-6851(01)00265-1 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15972477 | Plasmodium falciparum merozoite surface protein 8 is a ring-stage membraneprotein that localizes to the parasitophorous vacuole of infected erythrocytes. | Drew DR, Sanders PR, Crabb BS | 10.1128/IAI.73.7.3912-3922.2005 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 32738150 | Virus-like particles expressing Plasmodium berghei MSP-8 induce protectionagainst P. berghei infection | Lee SH, Chu KB, ..., Quan FS | 10.1111/pim.12781 |
| 28993460 | Evaluation of a Plasmodium-Specific Carrier Protein To Enhance Production ofRecombinant Pfs25, a Leading Transmission-Blocking Vaccine Candidate | Parzych EM, Miura K, ..., Burns JM Jr | 10.1128/IAI.00486-17 |
| 27347876 | Evolution of the Transmission-Blocking Vaccine Candidates Pvs28 and Pvs25 inPlasmodium vivax: Geographic Differentiation and Evidence of Positive Selection | Chaurio RA, Pacheco MA, ..., Escalante AA | 10.1371/journal.pntd.0004786 |
| 30975200 | Immunogenicity analysis of genetically conserved segments in Plasmodium ovalemerozoite surface protein-8 | Zhang X, Chu R, ..., Cheng Y | 10.1186/s13071-019-3412-0 |
| 35715803 | Cellular and antibody response in GMZ2-vaccinated Gabonese volunteers in acontrolled human malaria infection trial | Nouatin O, Ibanez J, ..., Mordmuller B | 10.1186/s12936-022-04169-8 |
| 27723436 | Transient Expression of Plasmodium berghei MSP8 and HAP2 in the Marine ProtozoanParasite Perkinsus marinus | Cold ER, Vasta GR, Robledo JA | 10.1645/16-88 |
| 26975721 | Immunogenicity of a chimeric Plasmodium falciparum merozoite surface proteinvaccine in Aotus monkeys | Burns JM Jr, Miura K, ..., Barnwell JW | 10.1186/s12936-016-1226-5 |
| 31533414 | Low Levels of Polymorphisms and Negative Selection in Plasmodum knowlesiMerozoite Surface Protein 8 in Malaysian Isolates | Atique Ahmed M, Kang HJ, Quan FS | 10.3347/kjp.2019.57.4.445 |
| 35214662 | Protection and Alleviated Inflammation Induced by Virus-like Particle VaccinesContaining Plasmodium berghei MSP-8, MSP-9 and RAP1 | Lee SH, Chu KB, Kang HJ, Quan FS | 10.3390/vaccines10020203 |
| 28532483 | Naturally acquired humoral and cellular immune responses to Plasmodium vivaxmerozoite surface protein 8 in patients with P. vivax infection | Cheng Y, Wang B, ..., Han ET | 10.1186/s12936-017-1837-5 |
| 22585960 | Evaluation of the immunogenicity and vaccine potential of recombinant Plasmodiumfalciparum merozoite surface protein 8 | Alaro JR, Angov E, ..., Burns JM Jr | 10.1128/IAI.00211-12 |
| 22414917 | Evidence of purifying selection on merozoite surface protein 8 (MSP8) and 10(MSP10) in Plasmodium spp | Pacheco MA, Elango AP, ..., Escalante AA | 10.1016/j.meegid.2012.02.009 |
| 24681218 | Differential T-cell responses of semi-immune and susceptible malaria subjects toin silico predicted and synthetic peptides of Plasmodium falciparum | Dinga JN, Kimbung Mbandi S, ..., Titanji VP | 10.1016/j.actatropica.2014.03.007 |
| 33199351 | A Chimeric Plasmodium vivax Merozoite Surface Protein Antibody Recognizes andBlocks Erythrocytic P. cynomolgi Berok Merozoites In Vitro | Shen FH, Ong JJY, ..., Cheng Y | 10.1128/IAI.00645-20 |