Product Description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.7.1.148 (4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0050515 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity |
| Process | GO:0008299 | isoprenoid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.11 | 0.94 |
| Trophozoite | expressed | 0.65 | 0.63 |
| Schizont | expressed | 0.15 | 0.90 |
| Gametocyte | expressed | 0.18 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0503100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.17 | MFS: -3.264 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1102800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I470
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.148 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | (4Z)-4-(furan-2-ylmethylidene)-3-phenyl-1,2-oxazol-5(4H)-one(4E)-3-methyl-4-[(5-phenylfuran-2-yl)methylidene]-1,2-oxazol-5(4H)-one(4Z)-4-[(5-methylfuran-2-yl)methylidene]-3-phenyl-1,2-oxazol-5(4H)-one6-(benzylsulfanyl)-2-(2-hydroxyphenyl)-4-oxo-3,4-dihydro-2H-1,3-thiazine-5-carbonitrileN-[3-(4-amino-1-benzyl-2-oxo-1,2-dihydropyrimidin-5-yl)prop-2-en-1-yl]ethanesulfonamideN-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]ethanesulfonamideN-[3-[4-amino-2-oxo-1-(1H-pyrazol-5-ylmethyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]ethanesulfonamideN-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]methanesulfonamideN-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]benzenesulfonamideethyl [4-amino-2-oxo-5-(3-[[(2,2,2-trifluoroethyl)sulfonyl]amino]prop-1-en-1-yl)pyrimidin-1(2H)-yl]acetateN-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]propane-1-sulfonamideN-[3-[4-amino-2-oxo-1-(tetrahydro-2-thienyl)-1,2-dihydropyrimidin-5-yl]prop-2-en-1-yl]cyclopropanesulfonamide... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8ZEY1 | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | Yersinia pestis | OrthoMCL, OrthoMCL BLAST | CHEMBL493923CHEMBL1835608 |
| P62615 | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | OrthoMCL | CHEMBL2289488CHEMBL3359036 | |
| P93841 | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic/chromoplastic | OrthoMCL BLAST | CHEMBL2289487 |
Protein Length: 537 | Molecular Weight (kDa): 63.376
UniProt ID(s): A0A1B1TK84, Q8I470
PDB ID(s): None
Isoelectric Point: 9.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004424 | 4-diphosphocytidyl-2C-methyl-D-erythritol kinase |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR036554 | GHMP kinase, C-terminal domain superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| Superfamily | SSF55060 | GHMP kinase, C-terminal domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 27 | 17 | 42 |
| disruptive | 20 | 57 | 42 | 103 |
| missense | 20 | 56 | 41 | 89 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 53 | 20 | 56 |
| disruptive | 27 | 155 | 82 | 124 |
| missense | 25 | 130 | 65 | 84 |
PlasmoDB Total SNPs: 118
Non-coding: 75 | Synonymous: 15 | Nonsynonymous: 28 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15279947 | Localization of organellar proteins in Plasmodium falciparum using a novel set oftransfection vectors and a new immunofluorescence fixation method. | Tonkin CJ, van Dooren GG, ..., McFadden GI | 10.1016/j.molbiopara.2004.05.009 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 38276186 | Investigating Novel IspE Inhibitors of the MEP Pathway in Mycobacterium | Choi SR, Narayanasamy P | 10.3390/microorganisms12010018 |
| 37324717 | A systematic overexpression approach reveals native targets to increase squaleneproduction in Synechocystis sp. PCC 6803 | Germann AT, Nakielski A, ..., Axmann IM | 10.3389/fpls.2023.1024981 |