Product Description: actin-depolymerizing factor 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0015629 | actin cytoskeleton |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0051015 | actin filament binding |
| Function | GO:0003785 | actin monomer binding |
| Process | GO:0030036 | actin cytoskeleton organization |
| Process | GO:0030042 | actin filament depolymerization |
| Process | GO:0051014 | actin filament severing |
| Process | GO:0048870 | cell motility |
| Process | GO:0060327 | cytoplasmic actin-based contraction involved in cell motility |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.87 | 0.69 |
| Trophozoite | expressed | 1.58 | 0.33 |
| Schizont | expressed | 2.17 | 0.15 |
| Gametocyte | expressed | 1.48 | 0.35 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0503400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.121 | MFS: -3.113 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1103100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I467
"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=0.26) with 3Q2B (Global RMSD=1.51)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100500
Most Similar Human Ortholog: P60981
TM-align score: 0.84 | RMSD: 1.85
Seq Identity: 0.29 | Length: 118 / 122
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000125868 | destrin, actin depolymerizing factor |
| ENSG00000165410 | cofilin 2 |
| ENSG00000172757 | cofilin 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 244
Non-coding: 241 | Synonymous: 3 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 122 | Molecular Weight (kDa): 13.741
UniProt ID(s): Q8I467
PDB ID(s): 2XF1
Isoelectric Point: 7.67
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002108 | Actin-depolymerising factor homology domain |
| InterPro | IPR017904 | ADF/Cofilin |
| PFam | PF00241 | Actin-depolymerising factor homology domain |
| Superfamily | SSF55753 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15975905 | A Plasmodium actin-depolymerizing factor that binds exclusively to actinmonomers. | Schuler H, Mueller AK, Matuschewski K | 10.1091/mbc.e05-02-0086 |
| 21628589 | Minimal requirements for actin filament disassembly revealed by structuralanalysis of malaria parasite actin-depolymerizing factor 1. | Wong W, Skau CT, ..., Baum J | 10.1073/pnas.1018927108 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24371134 | A mechanism for actin filament severing by malaria parasite actin depolymerizingfactor 1 via a low affinity binding interface. | Wong W, Webb AI, ..., Baum J | 10.1074/jbc.M113.523365 |
| 31180322 | Cooperation between tropomyosin and alpha-actinin inhibits fimbrin association withactin filament networks in fission yeast | Christensen JR, Homa KE, ..., Kovar DR | 10.7554/eLife.47279 |
| 34613787 | Counting actin in contractile rings reveals novel contributions of cofilin andtype II myosins to fission yeast cytokinesis | Malla M, Pollard TD, Chen Q | 10.1091/mbc.E21-08-0376 |