PF3D7_0503500

Genome location: Pf3D7_05_v3:142,470..145,987(-)

Genome classification: Core

Function and Localization

Product Description: protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.- (Protein-serine/threonine kinases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004672	protein kinase activity
Process	GO:0006468	protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	expressed	0.06	0.96
Schizont	expressed	0.12	0.91
Gametocyte	expressed	0.08	0.95

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0503500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.289 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1103200)

Relative Growth Rate: 1.01 ± 0.08
Confidence: 6.46

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1103200)

Modification: Disrupted | RMgm-2931

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I466

"Best" AlphaFill ligand hit: 4SP (, Local RMSD=3.03) with 1H1S (Global RMSD=3.50)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.-	Ex: cGMP-dependent protein kinase	NOATPKClDTTP53DRBADPH89PKIDT3NaFNaI...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102836

Most Similar Human Ortholog: E9PS17

TM-align score: 0.59 | RMSD: 4.23

Seq Identity: 0.21 | Length: 532 / 850

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000142186	SCY1 like pseudokinase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	52	17	40
disruptive	21	119	62	155
missense	18	117	59	143

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	85	29	45
disruptive	45	254	100	227
missense	41	230	84	145

PlasmoDB Total SNPs: 133

Non-coding: 56 | Synonymous: 54 | Nonsynonymous: 23 | Stop Codon: 0

Protein Information

Protein Length: 850 | Molecular Weight (kDa): 100.343

UniProt ID(s): Q8I466

PDB ID(s): None

Isoelectric Point: 7.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR016024	Armadillo-type fold
PFam	N/A	N/A
Superfamily	SSF48371	Armadillo-type fold
Superfamily	SSF56112	Protein kinase-like domain superfamily