Product Description: myosin B
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0015629 | actin cytoskeleton |
| Component | GO:0045177 | apical part of cell |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Component | GO:0031982 | vesicle |
| Function | GO:0005524 | ATP binding |
| Function | GO:0140657 | ATP-dependent activity |
| Function | GO:0003779 | actin binding |
| Function | GO:0051015 | actin filament binding |
| Function | GO:0030898 | actin-dependent ATPase activity |
| Function | GO:0000146 | microfilament motor activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0007015 | actin filament organization |
| Process | GO:0030050 | vesicle transport along actin filament |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.40 | 0.86 |
| Trophozoite | not expressed | 0.17 | 0.89 |
| Schizont | expressed | 0.51 | 0.81 |
| Gametocyte | not expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0503600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.99 | MFS: -2.544 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1103300)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PY17X_1104400)
Modification: Tagged | RMgm-5290
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I465
"Best" AlphaFill ligand hit: P15 (2,5,8,11,14,17-hexaoxanonadecan-19-ol, Local RMSD=0.02) with 6QDJ (Global RMSD=2.63)
No associated EC numbersOrthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_105053
Most Similar Human Ortholog: A0A590UK71
TM-align score: 0.87 | RMSD: 2.95
Seq Identity: 0.30 | Length: 605 / 801
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000196586 | myosin VI |
Protein Length: 801 | Molecular Weight (kDa): 93.178
PDB ID(s): None
Isoelectric Point: 6.86
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001609 | Myosin head, motor domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00063 | Myosin head, motor domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 38 | 24 | 62 |
| disruptive | 8 | 46 | 39 | 80 |
| missense | 8 | 46 | 39 | 70 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 84 | 35 | 43 |
| disruptive | 14 | 118 | 70 | 156 |
| missense | 13 | 100 | 61 | 104 |
PlasmoDB Total SNPs: 283
Non-coding: 241 | Synonymous: 29 | Nonsynonymous: 13 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25802338 | The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location inInvasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain. | Yusuf NA, Green JL, ..., Holder AA | 10.1074/jbc.M115.637694 |
| 30094538 | In vitro interaction between Plasmodium falciparum myosin B (PfMyoB) and myosin Atail interacting protein (MTIP). | Hernandez PC, Wasserman M, Chaparro-Olaya J | 10.1007/s00436-018-6039-8 |
| 36273584 | Analysis of Plasmodium falciparum myosin B ATPase activity and structure incomplex with the calmodulin-like domain of its light chain MLC-B | Pires I, Hung YF, ..., Kursula I | 10.1016/j.jbc.2022.102634 |
| 31283102 | Systematic analysis of Plasmodium myosins reveals differential expression,localisation, and function in invasive and proliferative parasite stages | Wall RJ, Zeeshan M, ..., Tewari R | 10.1111/cmi.13082 |
| 32554712 | The Functional Characterization of TcMyoF Implicates a Family ofCytostome-Cytopharynx Targeted Myosins as Integral to the Endocytic Machinery ofTrypanosoma cruzi | Chasen NM, Etheridge MG, Etheridge RD | 10.1128/mSphere.00313-20 |