Genome location: Pf3D7_05_v3:157,065..165,442(+)
Genome classification: Core
Product Description: cation transporting P-ATPase
SignalP Peptide: N/A
# Transmembrane Domains: 12
EC Numbers: 3.6.3.12 (Transferred entry: 7.2.2.6)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0043231 | intracellular membrane-bounded organelle |
| Function | GO:0015662 | P-type ion transporter activity |
| Process | GO:0006812 | cation transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.19 | 0.91 |
| Trophozoite | expressed | 1.17 | 0.43 |
| Schizont | expressed | 0.21 | 0.87 |
| Gametocyte | not expressed | 0.34 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0504000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.149 | MFS: -2.822 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1103600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PbANKA_1103600)
Modification: Disrupted | RMgm-4325
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I461
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.02) with 1T5T (Global RMSD=8.17)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.3.12 | K+-transporting ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100379
Most Similar Human Ortholog: B7WPN9
TM-align score: 0.80 | RMSD: 4.06
Seq Identity: 0.23 | Length: 1039 / 2393
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000127249 | ATPase 13A4 |
| ENSG00000133657 | ATPase 13A3 |
| ENSG00000159363 | ATPase cation transporting 13A2 |
| ENSG00000187527 | ATPase 13A5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 113 | 65 | 139 |
| disruptive | 106 | 282 | 150 | 367 |
| missense | 61 | 225 | 121 | 284 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 48 | 213 | 95 | 159 |
| disruptive | 174 | 602 | 257 | 555 |
| missense | 100 | 432 | 214 | 355 |
PlasmoDB Total SNPs: 372
Non-coding: 111 | Synonymous: 157 | Nonsynonymous: 103 | Stop Codon: 1
Protein Length: 2393 | Molecular Weight (kDa): 283.038
PDB ID(s): None
Isoelectric Point: 7.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001757 | P-type ATPase |
| InterPro | IPR008250 | P-type ATPase, A domain superfamily |
| InterPro | IPR018303 | P-type ATPase, phosphorylation site |
| InterPro | IPR023298 | P-type ATPase, transmembrane domain superfamily |
| InterPro | IPR023299 | P-type ATPase, cytoplasmic domain N |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF00122 | N/A |
| Superfamily | SSF56784 | HAD-like superfamily |
| Superfamily | SSF81653 | P-type ATPase, A domain superfamily |
| Superfamily | SSF81660 | P-type ATPase, cytoplasmic domain N |
| Superfamily | SSF81665 | P-type ATPase, transmembrane domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 11522345 | Characterization of P-type ATPase 3 in Plasmodium falciparum. | Rozmajzl PJ, Kimura M, ..., Meade JC | 10.1016/s0166-6851(01)00319-x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 28902970 | A genetic screen in rodent malaria parasites identifies five new apicoplastputative membrane transporters, one of which is essential in human malariaparasites | Sayers CP, Mollard V, ..., Goodman CD | 10.1111/cmi.12789 |
| 33485966 | Aminoglycerophospholipid flipping and P4-ATPases in Toxoplasma gondii | Chen K, Gunay-Esiyok O, ..., Gupta N | 10.1016/j.jbc.2021.100315 |