Product Description: ATP-dependent RNA helicase DDX27, putative
SignalP Peptide: MSFLLPLVIFFLFFFHLIKS
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Component | GO:0005730 | nucleolus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0004386 | helicase activity |
| Function | GO:0003729 | mRNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.54 | 0.69 |
| Schizont | expressed | 0.66 | 0.62 |
| Gametocyte | possibly expressed | 0.47 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0504200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.142 | MFS: -2.793 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1103800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I459
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.03) with 6L5M (Global RMSD=5.25)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 33 | 18 | 27 |
| disruptive | 9 | 35 | 28 | 61 |
| missense | 9 | 32 | 25 | 60 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 57 | 12 | 42 |
| disruptive | 22 | 92 | 48 | 115 |
| missense | 13 | 78 | 45 | 97 |
PlasmoDB Total SNPs: 127
Non-coding: 100 | Synonymous: 16 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 502 | Molecular Weight (kDa): 59.399
UniProt ID(s): Q8I459
PDB ID(s): None
Isoelectric Point: 10.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |