PF3D7_0504400

Genome location: Pf3D7_05_v3:172,042..174,574(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.1.3 (Deleted entry);3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005730	nucleolus
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Function	GO:0004386	helicase activity
Function	GO:0005515	protein binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.74	0.59
Schizont	expressed	0.21	0.87
Gametocyte	expressed	0.60	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0504400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.91 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1104000)

Relative Growth Rate: 0.09 ± 0.26
Confidence: 4.07

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I457

"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.01) with 2WAX (Global RMSD=3.08)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.3	adenosinetriphosphatase	No BRENDA inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102022

Most Similar Human Ortholog: Q9BQ39

TM-align score: 0.66 | RMSD: 4.13

Seq Identity: 0.31 | Length: 554 / 755

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000107625	DExD-box helicase 50
ENSG00000165732	DExD-box helicase 21

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	31	17	36
disruptive	12	56	24	56
missense	12	56	23	44

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	71	26	40
disruptive	30	101	40	98
missense	24	88	34	57

PlasmoDB Total SNPs: 68

Non-coding: 36 | Synonymous: 14 | Nonsynonymous: 18 | Stop Codon: 0

Protein Information

Protein Length: 755 | Molecular Weight (kDa): 87.598

UniProt ID(s): A7U5W8, Q8I457

PDB ID(s): None

Isoelectric Point: 9.64

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000629	ATP-dependent RNA helicase DEAD-box, conserved site
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR012562	GUCT
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF08152	GUCT
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
25204654	Kinase-associated endopeptidase 1 (Kae1) participates in an atypicalribosome-associated complex in the apicoplast of Plasmodium falciparum.	Mallari JP, Oksman A, Vaupel B, Goldberg DE	10.1074/jbc.M114.586735
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21