Product Description: 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.2.4.4 (3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005947 | mitochondrial alpha-ketoglutarate dehydrogenase complex |
| Component | GO:0030062 | mitochondrial tricarboxylic acid cycle enzyme complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003863 | 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity |
| Function | GO:0003826 | alpha-ketoacid dehydrogenase activity |
| Process | GO:0009083 | branched-chain amino acid catabolic process |
| Process | GO:0007584 | response to nutrient |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 1.00 |
| Trophozoite | expressed | 0.06 | 0.96 |
| Schizont | expressed | 0.14 | 0.89 |
| Gametocyte | expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0504600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.999 | MFS: -1.662 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1104200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1104200)
Modification: Disrupted | RMgm-2940
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I0X1
"Best" AlphaFill ligand hit: COI (alpha-ketoisocaproic acid, Local RMSD=0.02) with 1UMD (Global RMSD=0.88)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.2.4.4 | 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) | ATPNADHpyruvateClofibratemicroRNA 29amicroRNA 29b2-oxoglutarate2-oxobutanoate2-oxopentanoateisobutanoyl-CoAisopentanoyl-CoAcinnamylpyruvate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101601
Most Similar Human Ortholog: P21953
TM-align score: 0.85 | RMSD: 1.06
Seq Identity: 0.60 | Length: 329 / 381
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000083123 | branched chain keto acid dehydrogenase E1 subunit beta |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 22 | 11 | 23 |
| disruptive | 2 | 17 | 8 | 19 |
| missense | 2 | 17 | 7 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 48 | 20 | 14 |
| disruptive | 5 | 33 | 19 | 41 |
| missense | 2 | 29 | 19 | 28 |
PlasmoDB Total SNPs: 71
Non-coding: 61 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 381 | Molecular Weight (kDa): 43.032
UniProt ID(s): Q8I0X1
PDB ID(s): None
Isoelectric Point: 8.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005475 | Transketolase-like, pyrimidine-binding domain |
| InterPro | IPR009014 | Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II |
| InterPro | IPR029061 | Thiamin diphosphate-binding fold |
| InterPro | IPR033248 | Transketolase, C-terminal domain |
| PFam | PF02779 | Transketolase-like, pyrimidine-binding domain |
| PFam | PF02780 | Transketolase, C-terminal domain |
| Superfamily | SSF52518 | Thiamin diphosphate-binding fold |
| Superfamily | SSF52922 | Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |