PF3D7_0504900

Genome location: Pf3D7_05_v3:206,397..208,680(+)

Genome classification: Core

Function and Localization

Product Description: transmembrane protein TMEM222, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.04	0.98
Trophozoite	expressed	0.35	0.79
Schizont	expressed	0.36	0.79
Gametocyte	expressed	1.25	0.48

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0504900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.833 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4C5

"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=5.97) with 6Y4J (Global RMSD=20.07)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103330

Most Similar Human Ortholog: Q8TDQ4

TM-align score: 0.92 | RMSD: 1.62

Seq Identity: 0.38 | Length: 168 / 172

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000186501	transmembrane protein 222

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	10	2	15
disruptive	0	3	1	7
missense	0	3	1	7

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	19	7	16
disruptive	2	15	12	47
missense	2	10	10	39

PlasmoDB Total SNPs: 191

Non-coding: 185 | Synonymous: 5 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 172 | Molecular Weight (kDa): 20.093

UniProt ID(s): C0H4C5

PDB ID(s): None

Isoelectric Point: 8.59

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008496	TMEM222/RTE1
PFam	PF05608	TMEM222/RTE1
Superfamily	N/A	N/A