About Gene List

PF3D7_0505100 (TRS85)

Genome location: Pf3D7_05_v3:219,562..230,001(+)

Genome classification: Core

Function and Localization

Product Description: trafficking protein particle complex subunit 8, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:1990072 TRAPPIII protein complex
Component GO:0031410 cytoplasmic vesicle
Component GO:0005634 nucleus
Component GO:0000407 phagophore assembly site
Process GO:0000045 autophagosome assembly
Process GO:0030242 autophagy of peroxisome
Process GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport
Process GO:0034497 protein localization to phagophore assembly site

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.51 0.76
Trophozoite not expressed 1.33 0.39
Schizont not expressed 1.10 0.51
Gametocyte not expressed 0.86 0.56

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0505100

Resistome Missense Mutations: K2664N

Resistome Compounds with Missense Mutations: Atovaquone

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.239 | MFS: -2.679 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532844

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 30 140 69 165
disruptive 90 371 214 522
missense 51 307 184 430

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 54 239 99 192
disruptive 170 734 351 707
missense 101 593 295 490

PlasmoDB Total SNPs: 412

Non-coding: 171 | Synonymous: 134 | Nonsynonymous: 107 | Stop Codon: 0

Protein Information

Protein Length: 2754 | Molecular Weight (kDa): 328.677

UniProt ID(s): Q8I451

PDB ID(s): None

Isoelectric Point: 7.45

Protein Domain Annotations:

Source Family ID Description
InterPro IPR024420 TRAPP III complex, Trs85
PFam PF12739 TRAPP III complex, Trs85
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
36929574 The P4-ATPase Drs2 interacts with and stabilizes the multisubunit tetheringcomplex TRAPPIII in yeast Pazos I, Puig-Tinto M, ..., Gallego O 10.15252/embr.202256134
32729986 Newly identified genes contribute to vanillin tolerance in Saccharomycescerevisiae Liang Z, Wang X, ..., Shen Y 10.1111/1751-7915.13643
25800836 Ypt1 and TRAPP interactions: optimization of multicolor bimolecular fluorescencecomplementation in yeast Lipatova Z, Kim JJ, Segev N 10.1007/978-1-4939-2569-8_9
36519157 Investigation the global effect of rare earth gadolinium on the buddingSaccharomyces cerevisiae by genome-scale screening Cao Y, Zhang C, ..., Wang X 10.3389/fmicb.2022.1022054
22426882 Modular TRAPP complexes regulate intracellular protein trafficking throughmultiple Ypt/Rab GTPases in Saccharomyces cerevisiae Zou S, Liu Y, ..., Segev N 10.1534/genetics.112.139378
25581738 Bet3 participates in autophagy through GTPase Ypt1 in Saccharomyces cerevisiae Zou S, Liu Y, ..., Liang Y 10.1002/cbin.10416
22999853 Functional roles of YPT31 and YPT32 in clotrimazole resistance of Saccharomycescerevisiae through effects on vacuoles and ATP-binding cassette transporter(s) Tsujimoto Y, Takase D, ..., Matsui H 10.1016/j.jbiosc.2012.08.011