About Gene List

PF3D7_0505500 (MSH6)

Genome location: Pf3D7_05_v3:238,878..243,954(-)

Genome classification: Core

Function and Localization

Product Description: DNA mismatch repair protein MSH6, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0032301 MutSalpha complex
Component GO:0032300 mismatch repair complex
Component GO:0005634 nucleus
Function GO:0008094 ATP-dependent activity, acting on DNA
Function GO:0003684 damaged DNA binding
Function GO:0000400 four-way junction DNA binding
Function GO:0030983 mismatched DNA binding
Process GO:0006281 DNA repair
Process GO:0036297 interstrand cross-link repair
Process GO:0043570 maintenance of DNA repeat elements
Process GO:0000710 meiotic mismatch repair
Process GO:0006298 mismatch repair
Process GO:0045910 negative regulation of DNA recombination
Process GO:0006290 pyrimidine dimer repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.13 0.94
Trophozoite expressed 0.82 0.56
Schizont expressed 0.31 0.81
Gametocyte expressed 0.47 0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0505500

Resistome Missense Mutations: P462R

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.696 | MFS: -2.407 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1105100)

  • Relative Growth Rate: 0.11 ± 0.16
  • Confidence: 5.11

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I447

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.17) with 2WTU (Global RMSD=5.87)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100652

Most Similar Human Ortholog: A0A494C0M1

TM-align score: 0.70 | RMSD: 4.50

Seq Identity: 0.30 | Length: 979 / 1350

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000116062 mutS homolog 6

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 12 74 48 76
disruptive 21 69 53 100
missense 16 61 48 82

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 19 151 53 94
disruptive 42 160 89 198
missense 32 121 74 135

PlasmoDB Total SNPs: 157

Non-coding: 102 | Synonymous: 31 | Nonsynonymous: 23 | Stop Codon: 1

Protein Information

Protein Length: 1350 | Molecular Weight (kDa): 156.44

UniProt ID(s): Q8I447

PDB ID(s): None

Isoelectric Point: 6.35

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000432 DNA mismatch repair protein MutS, C-terminal
InterPro IPR007695 DNA mismatch repair protein MutS-like, N-terminal
InterPro IPR007696 DNA mismatch repair protein MutS, core
InterPro IPR007861 DNA mismatch repair protein MutS, clamp
InterPro IPR016151 DNA mismatch repair protein MutS, N-terminal
InterPro IPR017261 DNA mismatch repair protein MutS/MSH
InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
InterPro IPR036187 DNA mismatch repair protein MutS, core domain superfamily
InterPro IPR036678 MutS, connector domain superfamily
PFam PF00488 DNA mismatch repair protein MutS, C-terminal
PFam PF01624 DNA mismatch repair protein MutS-like, N-terminal
PFam PF05190 DNA mismatch repair protein MutS, clamp
PFam PF05192 DNA mismatch repair protein MutS, core
Superfamily SSF48334 DNA mismatch repair protein MutS, core domain superfamily
Superfamily SSF52540 P-loop containing nucleoside triphosphate hydrolase
Superfamily SSF53150 MutS, connector domain superfamily
Superfamily SSF55271 DNA mismatch repair protein MutS, N-terminal

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
38315739 Nonhomologous tails direct heteroduplex rejection and mismatch correction duringsingle-strand annealing in Saccharomyces cerevisiae Sapede E, Sugawara N, ..., Haber JE 10.1371/journal.pgen.1010527