Product Description: subtilisin-like protease 3
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.21.62 (Subtilisin)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Component | GO:0005737 | cytoplasm |
Component | GO:0005615 | extracellular space |
Component | GO:0016020 | membrane |
Function | GO:0005515 | protein binding |
Function | GO:0004252 | serine-type endopeptidase activity |
Process | GO:0016485 | protein processing |
Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | expressed | N/A | N/A |
Ring | possibly expressed | 0.13 | 0.93 |
Trophozoite | expressed | 0.22 | 0.88 |
Schizont | expressed | 1.52 | 0.34 |
Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0507200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.379 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I430
"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=0.41) with 7AM6 (Global RMSD=3.46)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
3.4.21.62 | Subtilisin | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
---|---|---|---|---|
Q7KAN4 | Subtilisin-like protease 1 | Plasmodium falciparum | OrthoMCL BLAST | CHEMBL3310712CHEMBL3310822CHEMBL3310709CHEMBL3326345... |
Protein Length: 769 | Molecular Weight (kDa): 88.047
UniProt ID(s): Q8I430
PDB ID(s): None
Isoelectric Point: 9.49
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000209 | Peptidase S8/S53 domain |
InterPro | IPR015500 | Peptidase S8, subtilisin-related |
InterPro | IPR022398 | Peptidase S8, subtilisin, His-active site |
InterPro | IPR023827 | Peptidase S8, subtilisin, Asp-active site |
InterPro | IPR036852 | Peptidase S8/S53 domain superfamily |
PFam | PF00082 | Peptidase S8/S53 domain |
Superfamily | SSF52743 | Peptidase S8/S53 domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 6 | 29 | 12 | 53 |
disruptive | 37 | 81 | 44 | 93 |
missense | 13 | 67 | 38 | 77 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 9 | 55 | 25 | 59 |
disruptive | 62 | 145 | 68 | 125 |
missense | 26 | 116 | 54 | 99 |
PlasmoDB Total SNPs: 146
Non-coding: 41 | Synonymous: 58 | Nonsynonymous: 47 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
19214190 | A multifunctional serine protease primes the malaria parasite for red blood cellinvasion. | Koussis K, Withers-Martinez C, ..., Blackman MJ | 10.1038/emboj.2009.22 |
22285468 | Expression and characterization of catalytic domain of Plasmodium falciparumsubtilisin-like protease 3. | Alam A, Bhatnagar RK, Chauhan VS | 10.1016/j.molbiopara.2011.12.009 |
24080030 | Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 onparasite profilin, a multifunctional protein. | Alam A, Bhatnagar RK, ..., Chauhan VS | 10.1016/j.molbiopara.2013.09.006 |
28351685 | Transcript and protein expression analysis of proteases in the blood stages ofPlasmodium falciparum. | Weissbach T, Golzmann A, ..., Julius Ngwa C | 10.1016/j.exppara.2017.03.006 |
30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |