About Gene List

PF3D7_0507200 (SUB3)

Genome location: Pf3D7_05_v3:298,070..301,000(-)

Genome classification: Core

Function and Localization

Product Description: subtilisin-like protease 3

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.21.62 (Subtilisin)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005737 cytoplasm
Component GO:0005615 extracellular space
Component GO:0016020 membrane
Function GO:0005515 protein binding
Function GO:0004252 serine-type endopeptidase activity
Process GO:0016485 protein processing
Process GO:0006508 proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring possibly expressed 0.13 0.93
Trophozoite expressed 0.22 0.88
Schizont expressed 1.52 0.34
Gametocyte expressed 0.20 0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0507200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.379 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I430

"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=0.41) with 7AM6 (Global RMSD=3.46)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.4.21.62 Subtilisin BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
Q7KAN4 Subtilisin-like protease 1 Plasmodium falciparum OrthoMCL BLAST CHEMBL3310712CHEMBL3310822CHEMBL3310709CHEMBL3326345...

Orthology Information

Ortholog Group (OrthoMCL): OG6_532299

No human ortholog(s)

Protein Information

Protein Length: 769 | Molecular Weight (kDa): 88.047

UniProt ID(s): Q8I430

PDB ID(s): None

Isoelectric Point: 9.49

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000209 Peptidase S8/S53 domain
InterPro IPR015500 Peptidase S8, subtilisin-related
InterPro IPR022398 Peptidase S8, subtilisin, His-active site
InterPro IPR023827 Peptidase S8, subtilisin, Asp-active site
InterPro IPR036852 Peptidase S8/S53 domain superfamily
PFam PF00082 Peptidase S8/S53 domain
Superfamily SSF52743 Peptidase S8/S53 domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 29 12 53
disruptive 37 81 44 93
missense 13 67 38 77

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 9 55 25 59
disruptive 62 145 68 125
missense 26 116 54 99

PlasmoDB Total SNPs: 146

Non-coding: 41 | Synonymous: 58 | Nonsynonymous: 47 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
19214190 A multifunctional serine protease primes the malaria parasite for red blood cellinvasion. Koussis K, Withers-Martinez C, ..., Blackman MJ 10.1038/emboj.2009.22
22285468 Expression and characterization of catalytic domain of Plasmodium falciparumsubtilisin-like protease 3. Alam A, Bhatnagar RK, Chauhan VS 10.1016/j.molbiopara.2011.12.009
24080030 Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 onparasite profilin, a multifunctional protein. Alam A, Bhatnagar RK, ..., Chauhan VS 10.1016/j.molbiopara.2013.09.006
28351685 Transcript and protein expression analysis of proteases in the blood stages ofPlasmodium falciparum. Weissbach T, Golzmann A, ..., Julius Ngwa C 10.1016/j.exppara.2017.03.006
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895