PF3D7_0507300 (SOPT)

Genome location: Pf3D7_05_v3:301,508..305,191(-)

Genome classification: Core

Function and Localization

Product Description: subtilisin-like ookinete protein SOPT

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0071944	cell periphery
Component	GO:0009986	cell surface
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.23	0.91
Trophozoite	possibly expressed	0.02	0.99
Schizont	expressed	0.21	0.90
Gametocyte	expressed	0.12	0.94

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0507300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.405 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1106900)

Modification: Disrupted | RMgm-4458

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I429

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533144

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	48	31	59
disruptive	53	174	75	210
missense	41	152	68	180

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	94	41	50
disruptive	95	312	137	253
missense	68	267	117	194

PlasmoDB Total SNPs: 217

Non-coding: 102 | Synonymous: 60 | Nonsynonymous: 55 | Stop Codon: 0

Protein Information

Protein Length: 893 | Molecular Weight (kDa): 107.039

UniProt ID(s): Q8I429

PDB ID(s): None

Isoelectric Point: 9.67

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
28559405	Plasmodium berghei P IMMS2 Promotes Ookinete Invasion of the Anopheles gambiae Mosquito Midgut	Ukegbu CV, Akinosoglou KA, Christophides GK, Vlachou D	10.1128/IAI.00139-17
29873127	Plasmodium falciparum subtilisin-like ookinete protein SOPT plays an importantand conserved role during ookinete infection of the Anopheles stephensi midgut.	Armistead JS, Jennison C, ..., Boddey JA	10.1111/mmi.13993
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g