Genome location: Pf3D7_05_v3:305,282..306,963(-)
Genome classification: Core
Product Description: subtilisin propeptide-like protein
SignalP Peptide: MKFLFAFNFFSLYIYLYEFLCIHLCGS
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0030414 | peptidase inhibitor activity |
| Function | GO:0004867 | serine-type endopeptidase inhibitor activity |
| Process | GO:0010951 | negative regulation of endopeptidase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.38 | 0.84 |
| Trophozoite | not expressed | 0.04 | 0.97 |
| Schizont | expressed | 0.29 | 0.85 |
| Gametocyte | not expressed | 0.03 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0507400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.998 | MFS: -0.799 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1107000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1107000)
Modification: Disrupted | RMgm-2953
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4D0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 8 | 8 | 3 |
| disruptive | 2 | 5 | 4 | 24 |
| missense | 2 | 5 | 4 | 23 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 20 | 5 | 9 |
| disruptive | 2 | 24 | 19 | 18 |
| missense | 2 | 19 | 16 | 14 |
PlasmoDB Total SNPs: 138
Non-coding: 126 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 153 | Molecular Weight (kDa): 17.892
UniProt ID(s): C0H4D0
PDB ID(s): None
Isoelectric Point: 8.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 31942933 | A malaria parasite subtilisin propeptide-like protein is a potent inhibitor ofthe egress protease SUB1. | Tarr SJ, Withers-Martinez C, ..., Blackman MJ | 10.1042/BCJ20190918 |